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. 2022 Jun 14;20(6):393.
doi: 10.3390/md20060393.

Secondary Metabolites and Biosynthetic Gene Clusters Analysis of Deep-Sea Hydrothermal Vent-Derived Streptomyces sp. SCSIO ZS0520

Huaran Zhang  1   2   3 , Yingying Chen  1 , Yanqing Li  1   2   3 , Yongxiang Song  1 , Junying Ma  1   2   3 , Jianhua Ju  1   2   3
Affiliations

Secondary Metabolites and Biosynthetic Gene Clusters Analysis of Deep-Sea Hydrothermal Vent-Derived Streptomyces sp. SCSIO ZS0520

Huaran Zhang et al. Mar Drugs. .

Abstract

Streptomyces sp. SCSIO ZS0520 is a deep-sea hydrothermal vent-derived actinomycete. Our previous metabolism investigation showed that Streptomyces sp. SCSIO ZS0520 is a producer of cytotoxic actinopyrones. Here, another four types of secondary metabolites were identified, including six salinomycin isomers (2-7), the macrolide elaiophylin (8), the triterpene N-acetyl-aminobacteriohopanetriol (9), and the pyrone minipyrone (10). Among them, compounds 2-6 and 10 are new compounds. To understand the biosynthetic pathway of these compounds, a bioinformatic analysis of the whole genome was carried out, which identified 34 secondary metabolite biosynthetic gene clusters. Next, the biosynthetic pathways responsive to four types of products were deduced on the basis of gene function predictions and structure information. Taken together, these findings prove the metabolite potential of ZS0520 and lay the foundations to solve the remaining biosynthetic issues in four types of marine natural products.

Keywords: Streptomyces; biosynthesis; deep sea; hydrothermal vent; polyketide.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Structures of compounds isolated from Streptomyces sp. SCSIO ZS0520 (* the absolute configuration of compounds 2 and 3 are interchangeable).
Figure 2
Figure 2
(a) The complete genomic graph of Streptomyces sp. SCSIO ZS0520. The six circles (from outer to inner) represent the size of the genome, CDs on positive chains, CDs on negative chains (different colors represent the functional classification of cogs with different CDs), rRNA and tRNA, GC content, and GC skew value; (b) the comparative genomics graph between Streptomyces sp. SCSIO ZS0520 and Streptomyces albus DSM 41398.
Figure 3
Figure 3
Proposed biosynthetic gene cluster (a) and pathway (b) of salinomycin.
Figure 4
Figure 4
Proposed biosynthetic gene cluster (a) and pathway (b) of elaiophilin.
Figure 5
Figure 5
Proposed biosynthetic gene cluster (a) and pathway (b) of N-acetyl-aminobacteriohopanetriol.
Figure 6
Figure 6
Proposed biosynthetic pathway of minipyrone.
See this image and copyright information in PMC

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