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. 2022:2508:9-17.
doi: 10.1007/978-1-0716-2376-3_2.

Culturing Suspension Cancer Cell Lines

Modeline N Longjohn  1 , Hong-Dien Phan  1 , Sherri L Christian  2
Affiliations

Culturing Suspension Cancer Cell Lines

Modeline N Longjohn et al. Methods Mol Biol. 2022.

Abstract

Suspension cell lines grow free-floating in the cell culture media without any attachment to the culture plate/vessel. Suspension cells typically mimic cells that exist in the circulation of multicellular animals such as mouse and humans. Generally, cell lines derived from the blood such as lymphocytes, megakaryocyte, and neutrophils grow in suspension. These cell lines can be used for experimental studies to understand the biology/biochemistry of cancer cells. In this chapter, procedures for working with suspension cell lines are provided, including protocols for thawing, culturing, and cryopreserving cancer cell lines. Importantly, this chapter demonstrates the best practices required to work with suspension cell lines, to minimize the risk of contaminations from adventitious microorganisms or from other cell lines.

Keywords: Cancer; Cell culture; Cell line; Cryopreservation; Culture media; Suspension cells.

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References

    1. Segeritz C-P, Vallier L (2017) Cell culture: growing cells as model systems in vitro. In: Jalali M, Saldanha FYL, Jalali M (eds) Basic science methods for clinical researchers. Elsevier, pp 151–172 - DOI
    1. Freshney RI (2016) Quantification. In: Capes-Davis A, Gregory C, Przyborski S (eds) Culture of animal cells: a manual of basic technique and specialized applications. Wiley, Hoboken, pp 483–512
    1. Ryu AH, Eckalbar WL, Kreimer A et al (2017) Use antibiotics in cell culture with caution: genome-wide identification of antibiotic-induced changes in gene expression and regulation. Sci Rep 7:1–9 - DOI
    1. Verhoeckx K, Cotter P, Kleiveland C et al (2015) General introduction to cells, cell lines and cell culture. In: Verhoeckx K, Cotter P, López-Expósito I et al (eds) The impact of food bioactives on health: in vitro and ex vivo models. Springer, Cham, pp 83–93
    1. Ackermann T, Tardito S (2019) Cell culture medium formulation and its implications in cancer metabolism. Trends Cancer 5:329–332 - DOI

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