Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2508:69-77.
doi: 10.1007/978-1-0716-2376-3_7.

Three-Dimensional Co-Culture Method for Studying Interactions Between Adipocytes, Extracellular Matrix, and Cancer Cells

Emmanuel C Asante  1 , Nikitha K Pallegar  1 , Alicia M Viloria-Petit  2 , Sherri L Christian  3
Affiliations

Three-Dimensional Co-Culture Method for Studying Interactions Between Adipocytes, Extracellular Matrix, and Cancer Cells

Emmanuel C Asante et al. Methods Mol Biol. 2022.

Abstract

Three-dimensional (D) culture models are increasingly becoming the model of choice for studying different biological phenomena such as cell-cell interaction, drug resistance, and gene expression. These models include extracellular matrix (ECM) proteins that better model the in vivo conditions as it allows cells to have both cell-cell and cell-ECM contacts. In the context of the tumor microenvironment, there are additional types of cells present in addition to the ECM. Thus, an intermediate between 2D cell culture and in vivo mouse models can be desired to interrogate the interactions between multiple cell types under the influence of the ECM. Here we describe a 3D co-culture technique for studying breast cancer-adipocyte interactions. This technique could easily be modified to analyze interactions between other cancer cell types and different fibroblast-like cells.

Keywords: 3D culture; Adipocytes; Breast cancer; Extracellular matrix; Immunofluorescence.

PubMed Disclaimer

References

    1. Ravi M, Paramesh V, Kaviya S, Anuradha E, Solomon FP (2015) 3D cell culture systems: advantages and applications. J Cell Physiol 230(1):16–26 - DOI
    1. Duval K, Grover H, Han L-H, Mou Y et al (2017) Modeling physiological events in 2D vs. 3D cell culture. Physiology 32(4):266–277 - DOI
    1. Kenny PA, Lee GY, Myers CA, Neve RM et al (2007) The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression. Mol Oncol 1(1):84–96 - DOI
    1. Luca AC, Mersch S, Deenen R, Schmidt S et al (2013) Impact of the 3D microenvironment on phenotype, gene expression, and EGFR inhibition of colorectal cancer cell lines. PLoS One 8(3):e59689 - DOI
    1. Aljitawi OS, Li D, Xiao Y, Zhang D et al (2014) A novel three-dimensional stromal-based model for in vitro chemotherapy sensitivity testing of leukemia cells. Leuk Lymphoma 55(2):378–391 - DOI

Publication types

Substances

LinkOut - more resources