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. 2022:2508:261-278.
doi: 10.1007/978-1-0716-2376-3_19.

Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes

Jinhong Kim  1 , Paola A Marignani  2   3
Affiliations

Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes

Jinhong Kim et al. Methods Mol Biol. 2022.

Abstract

Single-cell RNA sequencing (scRNA-seq) is an innovative technology that can be used to characterize transcriptome heterogeneity at the single-cell resolution. The Fluidigm C1 is an automated system for preparing complementary deoxyribonucleic acid (cDNA) samples that have been synthesized and preamplified from single cells for next-generation sequencing (NGS) analysis. Herein, we detail a workflow and protocol for scRNA-seq analysis of 3'-end enriched cDNA libraries and introduce strategies for validation of differential gene expression.

Keywords: Differential gene expression; Fluidigm C1 systems; Protein expression analysis; Validation; qRT-PCR; scRNA-seq.

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References

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