Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jun 9;12(6):805.
doi: 10.3390/biom12060805.

Analysis of Muscle Lipidome in Juvenile Rainbow Trout Fed Rapeseed Oil and Cochayuyo Meal

John Quiñones  1 , Rommy Díaz  1 , Jorge F Beltrán  2 , Lidiana Velazquez  3 , David Cancino  4 , Erwin Muñoz  5 , Patricio Dantagnan  6 , Adrián Hernández  6 , Néstor Sepúlveda  1   7 , Jorge G Farías  2
Affiliations

Analysis of Muscle Lipidome in Juvenile Rainbow Trout Fed Rapeseed Oil and Cochayuyo Meal

John Quiñones et al. Biomolecules. .

Abstract

This study aimed to analyze the effects on the lipidome of juvenile Oncorhynchus mykiss muscle fed 90% Brassica napus "rapeseed" oil and different amounts of Durvillaea antarctica "Cochayuyo" meal (1.5, 3 and 6%) as a replacement for cellulose. The analysis allowed for the identification of 329 lipids, mainly represented by phospholipids and fatty esters. The inclusion of Brassica napus oil significantly increased the levels of C18:2 species and fatty esters of hydroxylated fatty acids, which could play a bioactive role in human health. One of the most abundant lipids in all fillets was Phosphatidylcholine 33:6, which, according to the literature, could be considered a biomarker for the identification of Oncorhynchus mykiss. In all experimental diets, the species Phosphatidylethanolamine 15:1-18:24 showed four-fold higher levels than the control; increments of n-3- and n-6-rich phospholipids were also observed. Diets containing Durvillaea antarctica meal did not generate more significant variation in fish muscle phospholipids relative to the muscle of the rapeseed-oil-only group. These lipid species consist of medium- and long-chain fatty acids with different degrees of unsaturation. Still, it appears that the rapeseed oil masks the lipid contribution of the meal, possibly due to the low levels of total lipids in the macroalgae.

Keywords: LC-MS/MS; aquaculture; metabolomics; phospholipids; seaweed; sustainability.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Effect (p < 0.05%) of rapeseed oil (Brassica napus) and cochayuyo (Durvillaea antarctica) meal on the lipidome of juvenile rainbow trout (Oncorhynchus mykiss) muscle analyzed with LC-MS/MS. RO: rapeseed oil; 1.5%:1.5% cochayuyo meal; 3%:3% cochayuyo meal; 6%:6% cochayuyo meal.
Figure 2
Figure 2
A significant effect (p < 0.05) of rapeseed oil (Brassica napus) and cochayuyo (Duvillaea antarctica) meal on lipid species of juvenile rainbow trout (Oncorhynchus mykiss) muscle was determined with the Mann–Whitney U test. (A) 90% rapeseed oil (RO); (B) 90% of RO+1.5% cochayuyo meal; (C) 90% of RO+3% cochayuyo meal and (D) 90% of RO+6% cochayuyo meal. Lipids are presented in relation to their ion count abundance with respect to the control group.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Kromhout D., Bosschieter E.B., Coulander C.D. The inverse relation between fish consumption and 20-year mortality from coronary heart disease. N. Engl. J. Med. 1985;312:1205–1209. doi: 10.1056/NEJM198505093121901. - DOI - PubMed
    1. Streppel M.T., Ocké M.C., Boshuizen H.C., Kok F.J., Kromhout D. Long-term fish consumption and n-3 fatty acid intake in relation to (sudden) coronary heart disease death: The Zutphen study. Eur. Heart J. 2008;29:2024–2030. doi: 10.1093/eurheartj/ehn294. - DOI - PubMed
    1. Food and Agriculture Organization . The State of World Fisheries and Aquaculture 2018—Meeting the Sustainable Development Goals. Fisheries and Aquaculture Department; Rome, Italy: 2018.
    1. Naylor R.L., Hardy R.W., Buschmann A.H., Bush S.R., Cao L., Klinger D.H., Little D.C., Lubchenco J., Shumway S.E., Troell M. A 20-year retrospective review of global aquaculture. Nature. 2021;591:551–563. doi: 10.1038/s41586-021-03308-6. - DOI - PubMed
    1. Liu Z.-L., Zhao W., Hu W.-S., Zhu B., Xie J.-J., Liu Y.-J., Tian L.-X., Niu J. Lipid metabolism, growth performance, antioxidant ability and intestinal morphology of rainbow trout (Oncorhynchus mykiss) under cage culture with flowing water were affected by dietary lipid levels. Aquac. Rep. 2021;19:100593. doi: 10.1016/j.aqrep.2021.100593. - DOI

Publication types

LinkOut - more resources