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. 1978 Sep;135(3):1070-9.
doi: 10.1128/jb.135.3.1070-1079.1978.

Transconjugant analysis: limitations on the use of sequence-specific endonucleases for plasmid identification

S C Causey, L R Brown

Transconjugant analysis: limitations on the use of sequence-specific endonucleases for plasmid identification

S C Causey et al. J Bacteriol. 1978 Sep.

Abstract

We used the sequence-specific endonucleases EcoRI, SmaI, BamHI, HsuI, and HaeIII as identification tools in following the conjugal transfer of the well-studied R plasmids Sa, R388, RP4, and R6K. Transfers were both intergeneric and intrageneric. Plasmid fingerprints were generated from both single- and combination-enzyme digests. The Sa transconjugants yielded plasmids showing consistent fingerprints for each of the respective endonucleases used, whereas the three other R-plasmid transconjugants showed fingerprint changes.

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