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. 2022 Jun 10;11(12):3351.
doi: 10.3390/jcm11123351.

Differential Expression of Tissular miRNA-155 in Pediatric Gastritis

Săsăran Maria Oana  1 , Bănescu Claudia  2 , Riza Anca Lelia  3 , Mocan Simona  4 , Cârstea Claudia  2 , Dobru Ecaterina Daniela  5
Affiliations

Differential Expression of Tissular miRNA-155 in Pediatric Gastritis

Săsăran Maria Oana et al. J Clin Med. .

Abstract

Background: MicroRNA molecules, among them the intensely studied miRNA-155 (miR-155), are regarded as potential biomarkers of chronic gastric inflammation and premalignant lesion progression. However, literature data are scarce in terms of pediatric studies and in the evaluation of the predictive role of miRNA in early gastric inflammation. This study aims to assess the differential expression of miR-155 in relation to pediatric gastritis.

Methods: The present research was conducted on 192 patients with chronic dyspeptic symptoms who underwent upper digestive endoscopy. Bioptic samples were harvested for histopathological analysis and tissue miR-155 depiction. MiR-155 expression analysis was carried out through quantitative real-time polymerase chain reaction (qRT-PCR). The study population was divided into two groups: controls (93 patients) and study group (99 patients) with inflammatory modifications.

Results: MiR-155 expression was augmented in patients with gastritis but did not differ significantly from controls (p = 0.16). An increase in miR-155 expression was noted in relation to chronic gastritis, H. pylori infection, or increase in gastritis severity, but these variations were not important (p = 0.30, p = 0.44, and p = 0.45, respectively).

Conclusions: According to our study, pediatric gastritis increases, but does not greatly influence, miR-155 expression. Dynamic evaluation of miR-155 might enlighten its prognostic role in pediatric gastritis.

Keywords: Helicobacter pylori; biomarker; chronic gastritis; miR-155; pediatric gastritis.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
MiR-155 expression comparison between patients with gastritis and control group.
Figure 2
Figure 2
MiR-155 expression comparison between patients with H. pylori gastritis, non-H. pylori gastritis, and control group.
Figure 3
Figure 3
Log2(2−ΔΔCt) miR-155 comparison between patients with H. pylori gastritis and non-H. pylori gastritis.
Figure 4
Figure 4
MiR-155 expression comparison between patients with acute gastritis, chronic gastritis, and control group.
Figure 5
Figure 5
Log2(2−ΔCt) miR-155 comparison between patients with acute and chronic gastritis.
Figure 6
Figure 6
MiR-155 expression comparison between patients with severe gastritis, moderate gastritis, mild gastritis, and control group.
Figure 7
Figure 7
Log2(2−ΔCt) miR-155 comparison between patients with severe, moderate, and mild gastritis.
See this image and copyright information in PMC

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