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. 2022 Jun 17:14:797-804.
doi: 10.2147/IJWH.S361859. eCollection 2022.

Relationship of Placental and Serum Lipoprotein-Associated Phospholipase A2 Levels with Hypertensive Disorders of Pregnancy

Jing Wang  1 , Xing Dong  2 , Hong-Yan Wu  1 , Wen-Hua Bu  1 , Rong Cong  1 , Xin Wang  1 , Li-Xin Shang  1 , Wen Jiang  1
Affiliations

Relationship of Placental and Serum Lipoprotein-Associated Phospholipase A2 Levels with Hypertensive Disorders of Pregnancy

Jing Wang et al. Int J Womens Health. .

Abstract

Background: A series of studies has shown that lipoprotein-associated phospholipase A2 (Lp-PLA2) is closely associated with abnormal lipid metabolism and vascular endothelial cell injury, but its role in hypertensive disorders of pregnancy (HDP) remains unclear. This study aims to determine the relationship between placental and serum LP-PLA2 levels and HDP, and to provide a feasible method for predicting HDP.

Methods: The placental and serum Lp-PLA2 levels of 63 patients with HDP (20, 25, and 18 cases with gestational hypertension, mild preeclampsia, and severe preeclampsia, respectively) and 20 women with normal pregnancies (control group) were measured via a combination of tissue microarray and immunohistochemistry, real-time quantitative RT-PCR and enzyme-linked immunosorbent assay (ELISA).

Results: 1) The gene and protein expression levels of placental LP-PLA2: the HDP group had significantly higher levels than those of the control group (P < 0.05). The mild preeclampsia group had significantly higher levels than those of the control group (P < 0.05); the severe preeclampsia group had significantly higher levels than those of the mild preeclampsia group (P < 0.05). 2) Serum levels of Lp-PLA2: the HDP group had significantly higher levels than those of the control group (P < 0.05). The Lp-PLA2 levels increased gradually with the progression of the HDP; there were significant differences in the four groups using pair-wise comparisons (P < 0.05). 3) Serum levels of LP-PLA2 were positively correlated with placental LP-PLA2 levels in the HDP group (r = 0.435, P < 0.05).

Conclusion: Elevated Lp-PLA2 levels may be associated with the occurrence of HDP, and changes of Lp-PLA2 levels in the maternal blood may be regarded as a monitoring indicator for this disease.

Keywords: hypertensive disorders of pregnancy; lipoprotein-associated phospholipase A2; placenta; preeclampsia; serum.

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Conflict of interest statement

The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
TMA samples preparation and immunohistochemical staining of the Lp-PLA2 expression in trophoblast cells and decidual cells from placentae. (A) Expression of Lp-PLA2 in SPE villous trophoblastic tissue (a membrane, b cytoplasm). (B) Expression of Lp-PLA2 in SPE decidual tissue (a membrane, b cytoplasm). (C) Expression of Lp-PLA2 in MPE villous trophoblastic tissue. (D) Expression of Lp-PLA2 in MPE decidual tissue. (E) Expression of Lp-PLA2 in normal villous trophoblastic tissue. (F) Expression of Lp-PLA2 in normal decidual tissue. Original magnification X400.
Figure 2
Figure 2
Real-time quantitative PCR analysis of the LP-PLA2 levels in each group of placentas. *P<0.01 compared with the control group. **P<0.01 compared with the MPE group.
See this image and copyright information in PMC

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References

    1. Tarca AL, Romero R, Benshalom-Tirosh N, et al. The prediction of early preeclampsia: results from a longitudinal proteomics study. PLoS One. 2019;14(6):e0217273. doi:10.1371/journal.pone.0217273 - DOI - PMC - PubMed
    1. Jia K, Ma LJ, Wu SY, et al. Serum levels of complement factors C1q, Bb, and H in normal pregnancy and severe pre-eclampsia. Med Sci Monit. 2019;25(9):7087–7093. doi:10.12659/MSM.915777 - DOI - PMC - PubMed
    1. Tomimatsu T, Mimura K, Matsuzaki S, et al. Preeclampsia: maternal systemic vascular disorder caused by generalized endothelial dysfunction due to placental antiangiogenic factors. Int J Mol Sci. 2019;20(17):4246–4264. doi:10.3390/ijms20174246 - DOI - PMC - PubMed
    1. Olaoye T, Oyerinde OO, Elebuji OJ, et al. Knowledge, perception and management of pre-eclampsia among health care providers in a maternity hospital. Int J MCH AIDS. 2019;8(2):80–88. doi:10.21106/ijma.275 - DOI - PMC - PubMed
    1. Alahakoon TI, Medbury HJ, Williams H, et al. Lipid profiling in maternal and fetal circulations in preeclampsia and fetal growth restriction-a prospective case control observational study. BMC Pregnancy Childbirth. 2020;20(1):61–71. doi:10.1186/s12884-020-2753-1 - DOI - PMC - PubMed

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