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Case Reports
. 2022 Jun 24:64:e48.
doi: 10.1590/S1678-9946202264048. eCollection 2022.

Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022

Ingra Morales Claro  1   2 , Camila Malta Romano  1 , Darlan da Silva Candido  1   2   3 , Evelyn Lepka de Lima  4 , José Angelo Lauletta Lindoso  1   4   5 , Mariana Severo Ramundo  1 , Filipe Romero Rebello Moreira  2   6 , Luiz Alberto Costa Barra  4 , Luciana Marques Sansão Borges  4 , Lucas Alberto Medeiros  4 , Marcia Y S Tomishige  4 , Tomas Moutinho  5 , Anderson José Dias da Silva  4 , Camila Cristina Martini Rodrigues  4 , Luiz Cesar Fernandes de Azevedo  4 , Lucy Santos Villas-Boas  1 , Camila Alves Maia da Silva  1 , Thaís Moura Coletti  1 , Erika R Manuli  1 , Aine O'Toole  7 , Joshua Quick  8 , Nicholas Loman  8 , Andrew Rambaut  7 , Nuno R Faria  1   2   3 , Claudia Figueiredo-Mello  4 , Ester Cerdeira Sabino  1
Affiliations
Case Reports

Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022

Ingra Morales Claro et al. Rev Inst Med Trop Sao Paulo. .

Abstract

Monkeypox virus (MPXV), a zoonotic virus endemic to the African continent, has been reported in 33 non-endemic countries since May 2022. We report an almost complete genome of the first confirmed case of MPXV in Brazil. Shotgun metagenomic sequencing was completed in 18 hours, from DNA extraction to consensus sequence generation.

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Figures

Figure 1
Figure 1. Maximum likelihood phylogeny with 103 whole genome MPXV sequences, including 102 available in NCBI GenBank up to June 9, 2022 (see Supplementary Table S1 for the Accession Numbers used in this study). Highlighted genomes (yellow tips), including the SP01 described in this study (red tip), belong to the newly proposed B.1 lineage. An interactive visualization of the phylogeny presented here can be found at Argimón et al.
See this image and copyright information in PMC

References

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