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. 2022 Sep;119(9):2494-2504.
doi: 10.1002/bit.28163. Epub 2022 Jul 6.

Analyzing the effect of using axial impellers in large-scale bioreactors

Sören Bernauer  1   2 , Philipp Eibl  3   4 , Christian Witz  4 , Johannes Khinast  3 , Timo Hardiman  1   5
Affiliations

Analyzing the effect of using axial impellers in large-scale bioreactors

Sören Bernauer et al. Biotechnol Bioeng. 2022 Sep.

Abstract

In high-performance industrial fermentation processes, stirring and aeration may account for significant production costs. Compared to the widely applied Rushton impellers, axial-pumping impellers are known to yield a lower power draw and at the same time improve mixing. However, their lower gas dispersion capability requires stronger agitation, compromising these benefits. Diverse advanced impeller forms have been developed to cope with this challenge. We apply alternating radial and axial impellers and demonstrate strong gas dispersion and energy-efficient mixing for the first time in a large-scale (160 m3 ) bioreactor, based on experimental and computational fluid dynamics simulation data. For equal operating conditions (stirrer speed, aeration rate), this setup yielded similar gas hold-ups and better mixing times (35%) compared to a classical Rushton-only configuration. Hence, applying a radial impeller on an upper level for improving gas dispersion maintains the benefits of axial impellers in terms of reducing energy demand (up to 50%). We conclude that this effect is significant only at large-scale, when bubbles substantially expand due to the release of the hydrostatic pressure and have time to coalesce. The work thus extends current knowledge on mixing and aeration of large-scale reactors using classical impeller types.

Keywords: CFD simulation; gas expansion; gas hold-up; mixing time; multi-stage; pitched-blade impellers.

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References

REFERENCES

    1. Abrardi, V., Rovero, G., Baldi, G., Sicardi, S., & Conti, R. (1990). Hydrodynamics of a gas-liquid reactor stirred with a multi-impeller system. Chemical Engineering Research & Design, 68(6), 516-522.
    1. Arjunwadkar, S. J., Saravanan, K., Pandit, A. B., & Kulkarni, P. R. (1998). Optimizing the impeller combination for maximum hold-up with minimum power consumption. Biochemical Engineering Journal, 1(1), 25-30. https://doi.org/10.1016/S1369-703X(97)00005-3
    1. Bernauer, S., Schopf, M., Eibl, P., Witz, C., Khinast, J., & Hardiman, T. (2021). Characterization of the gas dispersion behavior of multiple impeller stages by flow regime analysis and CFD simulations. Biotechnology and Bioengineering, 118(8), 3058-3068. https://doi.org/10.1002/bit.27815
    1. Bernauer, S., Schopf, M., Khinast, J., & Hardiman, T. (2021). Scale-up of aerated industrial multistage rushton impeller bioreactors with complex rheology. Authorea, 118, 3058-3068.
    1. Brown, D. A. R., Jones, P. N., Middleton, J. C., Papadopoulos, G., & Arik, E. B. (2004). Experimental Methods, In Handbook of Industrial Mixing (pp. 145-256). John Wiley & Sons, Inc

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