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. 2022 May 14:3:287-292.
doi: 10.1016/j.ijregi.2022.05.003. eCollection 2022 Jun.

Prevalence of non-tuberculous mycobacteria among previously treated TB patients in the Gulf of Guinea, Africa

B D Thumamo Pokam  1   2 , D Yeboah-Manu  2 , S Ofori  2 , P W Guemdjom  3 , P M Teyim  4 , L Lawson  5 , D Amiteye  6 , N Y Yhiler  1   7 , I C Djuikoue  8   9 , A E Asuquo  10
Affiliations

Prevalence of non-tuberculous mycobacteria among previously treated TB patients in the Gulf of Guinea, Africa

B D Thumamo Pokam et al. IJID Reg. .

Abstract

Objective: Differentiation between non-tuberculous mycobacteria (NTM) and Mycobacterium tuberculosis complex (MTBC) is crucial for case management with the appropriate antimycobacterials. This study was undertaken in three West and Central African countries to understand NTM associated with pulmonary tuberculosis in the sub-region.

Methods: A collection of 503 isolates (158 from Cameroon, 202 from Nigeria and 143 from Ghana) obtained from solid and liquid cultures were analysed. The isolates were tested for drug susceptibility, and MTBC were confirmed using IS6110. All IS6110-negative isolates were identified by 65-kilodalton heat shock protein (hsp65) gene amplification, DNA sequencing and BLAST analysis.

Results: Overall, the prevalence of NTM was 16/503 (3.2%), distributed as 2/202 (1%) in Nigeria, 2/158 (1.3%) in Cameroon and 12/143 (8.4%) in Ghana. The main NTM isolates included 5/16 (31.3%) M. fortuitum, 2/16 (12.5%) M. intracellulare and 2/16 (12.5%) M. engbaekii. Eight (57.1%) of the 14 previously treated patients harboured NTM (odds ratio 0.21, 95% confidence interval 0.06-0.77; P=0.021). Three multi-drug-resistant strains were identified: M. engbaekii, M. fortuitum and M. intracellulare.

Conclusion: NTM were mainly found among individuals with unsuccessful treatment. This highlights the need for mycobacterial species differentiation using rapid molecular tools for appropriate case management, as most are resistant to routine first-line antimycobacterials.

Keywords: Drug resistance; NTM; Retreatment patients; West/Central Africa; hsp65 sequencing.

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Conflict of interest statement

None declared.

Figures

Figure 1
Figure 1
Distribution of non-tuberculous mycobacteria/non-mycobacteria isolates by country, drug resistance and previous treatment status. STR, streptomycin; INH, isoniazid; RIF, rifampicin; ETH, ethambutol; MDR, multi-drug resistant.
See this image and copyright information in PMC

References

    1. Aliyu G, El-Kamary SS, Abimiku A, Brown C, Tracy K, Hungerford L, et al. Prevalence of non-tuberculous mycobacterial infections among tuberculosis suspects in Nigeria. PLoS One. 2013;8:e63170. - PMC - PubMed
    1. Cadmus SI, Diarra B, Traore B, Maiga M, Siddiqui S, Tounkara A, et al. Nontuberculous mycobacteria isolated from tuberculosis suspects in Ibadan. Nigeria. J Pathogens. 2016;2016 - PMC - PubMed
    1. da Costa ARF, Falkinham JO, III, Lopes ML, Barretto AR, Felicio JS, Sales LHM, et al. Occurrence of nontuberculous mycobacterial pulmonary infection in an endemic area of tuberculosis. PLoS Negl Trop Dis. 2013;7:e2340. - PMC - PubMed
    1. Devallois A, Goh KS, Rastogi N. Rapid identification of mycobacteria to species level by PCR-restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species. J Clin Microbiol. 1997;35:2969–2973. - PMC - PubMed
    1. Egbe NF, Muwonge A, Ndip L, Kelly RF, Sander M, Tanya V, et al. Abattoir-based estimates of mycobacterial infections in Cameroon. Sci Rep. 2016;6:24320. - PMC - PubMed