Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jun 15:2022:3751521.
doi: 10.1155/2022/3751521. eCollection 2022.

Cross Protectivity Analysis of 49.8 kDa Pili Subunits of S. flexneri against Vibrio cholerae Infection

Dwi Yuni Nur Hidayati  1 , Septha Rully  2 , Aisyah Amalia  2 , Elsa Larissa Widyani  2 , Genitri Indraswari  2 , Adrian Prasetya  2 , Merika Soraya  2 , Sri Winarsih  1 , Sumarno Reto Prawiro  1
Affiliations

Cross Protectivity Analysis of 49.8 kDa Pili Subunits of S. flexneri against Vibrio cholerae Infection

Dwi Yuni Nur Hidayati et al. Interdiscip Perspect Infect Dis. .

Abstract

Background: Although the AMV and AMS vaccine candidates have similar characteristics as hemagglutinin and adhesive molecules, there are differences in molecular weight.

Objective: The research aims to determine the immunological cross-reaction between AMS and AMV.

Method: Antihemagglutination test used the anti-adhesion molecular antibody AMS. Next, we examined the immune response that has to be linked with protectivity. The model of the research uses MLIL. The sample separated the mice into four groups, and each group had five mice. The first group was the negative control group. The second group was given AMV and infected with Shigella flexneri. The third group was immunized with AMV before being exposed to Shigella flexneri. The last group was infected with Vibrio cholerae. The immune response results were evaluated by calculating the weight of MLIL and counting the colony of bacteria. We also examined other AMS immune responses, namely, β-defensin and s-IgA levels. To get the data, we measured the number of Th17 immune effector cells, T-reg, and proinflammatory cytokine IL-17A. Data analysis was performed using ANOVA, independent t-test, Kruskal-Wallis, and Mann-Whitney tests.

Results: An antihemagglutination cross immune response, intestinal weight, the number of bacterial colonies, and other findings were found to be significant (p < 0.05) for the levels of β-defensin, s-IgA, Th17, T-reg, and IL-17A.

Conclusion: The 49.8 kDa·MW protein subunit of the Shigella flexneri adhesion molecule could act as a candidate vaccine homologous for shigellosis and cholera in the future.

PubMed Disclaimer

Conflict of interest statement

The authors declare that there are no conflicts of interest in this study.

Figures

Figure 1
Figure 1
Profile of MW of protein pili subunits V. cholerae (a) and S. flexneri (b) used SDS-PAGE.
Figure 2
Figure 2
(a) The result of hemagglutination AMV and (b) the result of AMS antibody antihemagglutination AMS inhibited hemagglutination AMV.
Figure 3
Figure 3
Macroscopic intestinal weight changes of mice vaccinated with V. cholerae-infected MLIL method. (a) The first group was the negative control group. (b) The second group we infected with S. flexneri. (c) The third group was the same as the second group, but the sample group was infected with V. cholerae. (d) The fourth group was immunized before being affected by S. flexneri. (e) The last group is the same as the fourth group but infected with V. cholerae.
Figure 4
Figure 4
The graph result of weighting every piece of intestinal ligated in the sample.
Figure 5
Figure 5
The graph of the number of colony-forming units in the five groups.
Figure 6
Figure 6
Compared to control, the difference in secretory IgA and β-defensin is secondary to Shigella vaccination.
Figure 7
Figure 7
Scatter graph of Th17 proportion (pointed with a black circle) in the control group (a) and S. flexneri 49.8 kDa subunit pili vaccinated group (b). Scatter graph of T-regulatory proportion (pointed with black circles) in the control group (c) and S. flexneri 49.8 kDa subunit pili vaccinated group (d).
Figure 8
Figure 8
Profile of IL-17, Th17, Th-reg, and ratio Th17 T-reg.
See this image and copyright information in PMC

References

    1. Ali M., Nelson A. R., Lopez A. L., Sack D. A. Updated global burden of Cholera in endemic countries. PLoS Neglected Tropical Diseases . 2015;9(6) doi: 10.1371/journal.pntd.0003832.e0003832 - DOI - PMC - PubMed
    1. Kotloff K. L., Riddle M. S., Platts-Mills J. A., Pavlinac P., Zaidi A. K. M. Shigellosis. The Lancet . 2018;391(10122):801–812. doi: 10.1016/s0140-6736(17)33296-8. - DOI - PubMed
    1. Liu J., Platts-Mills J. A., Juma J., et al. Use of quantitative molecular diagnostic methods to identify causes of diarrhoea in children: a reanalysis of the GEMS case-control study. The Lancet . 2016;388(10051):1291–1301. doi: 10.1016/s0140-6736(16)31529-x. - DOI - PMC - PubMed
    1. Salyers A. A., Whitt D. D. A molecular approach. American Society for Microbiology . 2002;629
    1. Fitrianingsih A. A., Rachma L. N., Miliana A., Hernowati T. E., Aulanni’am A., Prawiro S. R. Cross reaction among antibody pili sub unit hemagglutinin proteins and outer membrane sub unit hemagglutinin proteins of Shigella flexneri. Journal of Tropical Life Science . 2017;7(1):1–7. doi: 10.11594/jtls.07.01.01. - DOI

LinkOut - more resources