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. 2021 Oct 28:1:142-149.
doi: 10.1016/j.ijregi.2021.10.007. eCollection 2021 Dec.

Bacterial drug-resistance patterns and genetic diversity of bacteria-associated bacteriuria in diabetic patients in Ghana

Akua Obeng Forson  1 , Dickson Agyei Menkah  1 , Marjorie Ntiwaa Quarchie  1 , Shittu Bunkunmi Dhikrullahi  2 , Michael Olu-Taiwo  1 , Francis Samuel Codjoe  1
Affiliations

Bacterial drug-resistance patterns and genetic diversity of bacteria-associated bacteriuria in diabetic patients in Ghana

Akua Obeng Forson et al. IJID Reg. .

Abstract

Objectives: Our study aimed to determine the etiology of urinary tract infections (UTIs), resistance profiles of isolated bacteria, and virulence factors of Escherichia coli associated with bacteriuria in diabetic patients in Ghana.

Methods: Midstream urine samples from 982 diabetic patients were tested for uropathogens at the National Diabetes Management and Research Centre in Ghana, using standard bacteriological methods, with antibiogram testing of the isolates using the Kirby-Bauer disk diffusion, as per CLSI guidelines. Polymerase chain reaction (PCR) was used to investigate the phylogenetic groupings and virulence factor (VF) genes of isolated E. coli.

Results: The overall prevalence of UTIs was 9.2%, and the main uropathogens were Klebsiella spp. (55.6%) and Escherichia coli (31.3%). Age, duration of diabetes, and a previous history of UTIs were risk factors associated with UTI (p-value < 0.05). High levels of antibacterial resistance to cefuroxime (84%), ampicillin (80%), and gentamicin (70.7%) were observed. The distribution of VFs in each phylogenetic group revealed that sfa-iutA-KpsTMII-KpsTMIII genes were associated with group B2, and iutA-ibe were associated with group D.

Conclusions: The isolated uropathogens were highly resistant, and the E. coli isolates possessed varying VFs. Continuous monitoring of bacteria associated with UTI in diabetics is highly recommended.

Keywords: Escherichia coli; Ghana; phylogenetic group; urinary tract infections (UTI); virulence factor.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Distribution of uropathogens and fasting plasma glucose (FPG) measurements in patients.
See this image and copyright information in PMC

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