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. 2022 Aug 31;10(4):e0067322.
doi: 10.1128/spectrum.00673-22. Epub 2022 Jun 28.

Spread of Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates Producing NDM-Type Metallo-β-Lactamase in Myanmar

Satomi Takei  1   2 , Yu Jie Lu  3 , Mari Tohya  3 , Shin Watanabe  4 , Shigeki Misawa  1 , Yoko Tabe  2 , Takashi Miida  2 , San Mya  5 , Htay Htay Tin  5 , Tatsuya Tada  3 , Teruo Kirikae  3
Affiliations

Spread of Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates Producing NDM-Type Metallo-β-Lactamase in Myanmar

Satomi Takei et al. Microbiol Spectr. .

Abstract

A total of 38 isolates of carbapenem-resistant Klebsiella pneumoniae harboring blaNDM were obtained during surveillance of 10 hospitals in Myanmar. Of these 38 isolates, 19 (50%) harbored genes encoding 16S rRNA methylases, such as armA or rmtB. The K. pneumoniae strains tested belonged to 17 sequence types (STs), including the high-risk clonal lineages ST101 and ST147. The ST101 and ST147 isolates carried IncFII plasmids harboring blaNDM-5 and IncFIB(pQil) plasmids harboring blaNDM-1, respectively. These results indicate that IncFII plasmids harboring blaNDM-5 and IncFIB(pQil) plasmids harboring blaNDM-1 have been spreading in K. pneumoniae ST101 and ST147 isolates, respectively, in Myanmar. IMPORTANCE The emergence of carbapenem-resistant K. pneumoniae has become a serious problem in medical settings worldwide. The present study demonstrated that carbapenem-resistant K. pneumoniae strains have been spreading in medical settings in Myanmar. In particular, plasmid genes encoding NDMs and 16S rRNA methylases have been spreading in K. pneumoniae high-risk clones.

Keywords: 16S rRNA methylase; Klebsiella pneumoniae; NDM-type metallo-β-lactamase; carbapenemase-producing Enterobacteriaceae.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIG 1
FIG 1
Phylogenetic tree of 38 carbapenem-resistant K. pneumoniae complex isolates obtained from clinical samples at 10 hospitals in Myanmar. The tree was constructed by the maximum likelihood method based on core-genome SNPs.
FIG 2
FIG 2
Genomic environments of blaNDM in K. pneumoniae complex strains isolated from various clinical samples obtained at 10 hospitals in Myanmar.
FIG 3
FIG 3
Genomic environments of armA and rmtB in K. pneumoniae complex strains isolated from various clinical samples obtained at 10 hospitals in Myanmar.
FIG 4
FIG 4
Comparison of the plasmid sequences of IncFIB(pQil), IncFII, IncX3, IncC, IncM2, and IncFIB(pQil)/IncFII(K). The images were generated using BLAST Ring Image Generator software (https://sourceforge.net/projects/brig/files/BRIG-0.95-dist.zip/download). Plasmid sequences belonging to each Inc type were compared with plasmids of MyNCGM111 for IncFIB(pQil) (A), MyNCGM439 for IncFII (B), MyNCGM036 for IncX3 (C), MyNCGM076 for IncC (D), MyNCGM127 for IncM2 (E), and MyNCGM143 for IncFIB(pQil)/IncFII(K) (F).
See this image and copyright information in PMC

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