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. 2022 Sep;159(2):369-375.
doi: 10.1007/s11060-022-04071-y. Epub 2022 Jun 28.

Stimulated Raman histology facilitates accurate diagnosis in neurosurgical patients: a one-to-one noninferiority study

Evan H Einstein  1 , Faina Ablyazova  2 , Ashley Rosenberg  2 , Manju Harshan  3 , Samuel Wahl  3 , Gady Har-El  4 , Peter D Constantino  4 , Jason A Ellis  2 , John A Boockvar  2 , David J Langer  2 , Randy S D'Amico  2
Affiliations

Stimulated Raman histology facilitates accurate diagnosis in neurosurgical patients: a one-to-one noninferiority study

Evan H Einstein et al. J Neurooncol. 2022 Sep.

Abstract

Objective: Stimulated Raman histology (SRH) offers efficient and accurate intraoperative neuropathological tissue analysis without procedural alteration to the diagnostic specimen. However, there are limited data demonstrating one-to-one tissue comparisons between SRH and traditional frozen sectioning. This study explores the non-inferiority of SRH as compared to frozen section on the same piece of tissue in neurosurgical patients.

Methods: Tissue was collected over a 1-month period from 18 patients who underwent resection of central nervous system lesions. SRH and frozen section analyses were compared for diagnostic capabilities as well as assessed for quality and condition of tissue via a survey completed by pathologists.

Results: SRH was sufficient for diagnosis in 78% of specimens as compared to 94% of specimens by frozen section of the same specimen. A Fisher's exact test determined there was no significant difference in diagnostic capability between the two groups. Additionally, both quality of SRH and condition of tissue after SRH were deemed to be non-inferior to frozen section.

Conclusions: This study provides further evidence for the non-inferiority of SRH techniques. It is also the first study to demonstrate SRH accuracy using one-to-one tissue analysis in neuropathological specimens.

Keywords: Brain tumor pathology; NIO histology; Neurosurgical pathology; Tumor histology; Virtual pathology.

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