Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jun 14:13:935117.
doi: 10.3389/fphar.2022.935117. eCollection 2022.

Dose-Effect Relationship of Chitosan and Danshen Combined Injection for Fallopian Tube Recanalization

Chen Huang  1   2 , Qiong Wu  3   4 , Jiabin Liang  5 , Qian Wang  1 , Xueping He  1 , Yanqiu Xie  1 , Yanbing Lu  1 , Jianfen Su  1 , Yutuan Tang  1
Affiliations

Dose-Effect Relationship of Chitosan and Danshen Combined Injection for Fallopian Tube Recanalization

Chen Huang et al. Front Pharmacol. .

Abstract

Objectives: This study examined the dose-effect relationship of chitosan and danshen combined injections on the long-term prevention of fallopian tube re-obstructions, with increased pregnancy rates in infertile women. Methods: High-performance liquid chromatography was used to determine the content changes of combined chitosan and danshen injection. Two hundred and eighty patients with fallopian tube obstructions were randomly assigned to four groups. Group A (control group, saline), Group B (2 ml chitosan, 4 ml danshen), Group C (2 ml chitosan, 10 ml danshen), and Group D (1 ml chitosan, 10 ml danshen). Injections were administered after tubal recanalization. Results: The effective constituent of chitosan and danshen injection was stable. Tubal patency rate was 94.2% and 87.3% in Group C after 1 and 3 years, respectively, which was significantly higher than Groups A (38.6%, 31.5%), B (73.5%, 64.1%), and D (68.5%, 50.7%). Intrauterine pregnancy rates were 61.8% and 79.4% in Group C after 1 and 3 years, respectively, and were significantly higher than Groups A (31.8%, 34.8%), B (40.1%, 62.5%), and D (38.5%, 58.5%) (p < 0.05). Conclusion: Combined Chitosan and danshen injections prevented tubal obstruction and increased pregnancy rates for long periods using an optimal ratio of 1 part chitosan and 5 parts danshen.

Keywords: chitosan; danshen; dose-effect relationships; drug delivery; fallopian tube recanalization.

PubMed Disclaimer

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Chromatogram Chart. (A). The 210 nm wavelength shows the peak for carboxymethyl chitin (a). (B). The 280 nm wavelength shows peaks for salvianic acid A sodium (b) and protocatechualdehyde (c).
FIGURE 2
FIGURE 2
Flow chart illustrating patient recruitment and analysis.
FIGURE 3
FIGURE 3
Pre- and post-injection salpingography. (A). Control group. (A1), The right fallopian tube completely obstructed at the preoperative stage. (A2), The right fallopian tube partially unobstructed 1 day after interventional recanalization. (B). Chitosan and danshen combined injection group (Group 2, 1:2 ratio) (B1), The right fallopian tube completely obstructed at the pre-injection stage. (B2), The right fallopian tube unobstructed 1 day after interventional recanalization. (C). Chitosan and danshen combined injection group (Group C, 1:5 ratio) (C1), The left fallopian tube completely obstructed at the preoperative stage. (C2), The left fallopian tube unobstructed 1 day after interventional recanalization. (D). Chitosan and danshen combined injection group (Group D, 1:10 ratio) (D1), The left fallopian tube completely obstructed at the pre-injection stage. D2, The left fallopian tube unobstructed 1 day after interventional recanalization.
FIGURE 4
FIGURE 4
Selective salpingography showed fallopian tubes obstructed 1 year post-injection. (A), Control group; (B), Chitosan and Danshen combined injection group (1:2); (C), Chitosan and Danshen injection group (1:5); and (D), Chitosan and Danshen combined injection group (1:10). The yellow arrow shows the tube recanalized.
FIGURE 5
FIGURE 5
Selective salpingography showed fallopian tubes partially obstructed 3 years post-injection. (A), Control group; (B), Chitosan and Danshen combined injection group (1:2); (C), Chitosan and Danshen combined injection group (1:5); and (D), Chitosan and Danshen combined injection group (1:10). The blue arrow shows the tube partially recanalized.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Abolhasani A., Heidari F., Abolhasani H. (2020). Development and Characterization of Chitosan Nanoparticles Containing an Indanonic Tricyclic Spiroisoxazoline Derivative Using Ion-Gelation Method: an In Vitro Study. Drug Dev. Ind. Pharm. 46, 1604–1612. 10.1080/03639045.2020.1811304 - DOI - PubMed
    1. Anil G., Tay K. H., Loh S. F., Yong T. T., Ong C. L., Tan B. S. (2011). Fluoroscopy-guided, Transcervical, Selective Salpingography and Fallopian Tube Recanalisation. J. Obstet. Gynaecol. 31, 746–750. 10.3109/01443615.2011.593647 - DOI - PubMed
    1. Babicheva T. S., Konduktorova A. A., Shmakov S. L., Shipovskaya A. B. (2020). Formation of Liesegang Structures under the Conditions of the Spatiotemporal Reaction of Polymer-Analogous Transformation (Salt → Base) of Chitosan. J. Phys. Chem. B 124, 9255–9266. 10.1021/acs.jpcb.0c07173 - DOI - PubMed
    1. Cai H., Li G. (2020). Efficacy of Alginate-And Chitosan-Based Scaffolds on the Healing of Diabetic Skin Wounds in Animal Experimental Models and Cell Studies: A Systematic Review. Wound Repair Regen. 28, 751–771. 10.1111/wrr.12857 - DOI - PubMed
    1. Chung Y. J., An S. Y., Yeon J. Y., Shim W. S., Mo J. H. (2016). Effect of a Chitosan Gel on Hemostasis and Prevention of Adhesion after Endoscopic Sinus Surgery. Clin. Exp. Otorhinolaryngol. 9, 143–149. 10.21053/ceo.2015.00591 - DOI - PMC - PubMed

LinkOut - more resources