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. 2022 Jun 25:2022:9671594.
doi: 10.1155/2022/9671594. eCollection 2022.

Green Synthesis of Silver Nanoparticles Using the Tridax procumbens Plant Extract and Screening of Its Antimicrobial and Anticancer Activities

Rohini Pungle  1   2 , Shivraj Hariram Nile  3 , Nilesh Makwana  4 , Ragini Singh  5 , Rana P Singh  5 , Arun S Kharat  4
Affiliations

Green Synthesis of Silver Nanoparticles Using the Tridax procumbens Plant Extract and Screening of Its Antimicrobial and Anticancer Activities

Rohini Pungle et al. Oxid Med Cell Longev. .

Abstract

In this study, we report the green synthesis of silver nanoparticles (AgNPs) using the aqueous leaf extract of Tridax procumbens (TNP), which acts as the source of the reducing and capping agent. The distinctive absorption at 370 nm suggested synthesis of TNPs, which was confirmed by TEM, with a size in the range of 11.1 nm to 45.4 nm and a spherical shape, having a face-centered cubic structure, analyzed by XRD, and a Zeta potential of -20.7 mV, which indicated a moderate stability of TNP. The FTIR analysis revealed the presence of amines and hydroxyl groups with fluoro compounds over the TNPs. The HRLC-MS analysis of TNPs suggested the presence of a major capping agent such as fosinopril and reducing agents such as peptides (Gln Gly Ala, Ser Pro Asn, and Leu Met), terpenoids (lupanyl acid, tiamulin), polyphenol (peucenin), and alkaloids (8',10'-dihydroxydihydroergotamine, carteolol). The synthesized silver nanoparticles exhibited antimicrobial activity against multidrug-resistant (MDR) clinical isolates (Escherichia coli, Shigella spp., Aeromonas spp., Pseudomonas aeruginosa, and Candida tropicalis) and had anticancer activity against A459 (IC50 42.70 μg/ml). The extraction of partially purified aqueous leaf extracts by silica gel column chromatography followed by HPLC to synthesize silver nanoparticles (TNP11) and analyzed by HRLC-MS suggested that dipeptides were involved in the reduction of Ag+ to Ag0. Overall, the results showed that the green silver nanoparticles of T. procumbens could be safe, as they are endowed with potential antimicrobial activity against MDR clinical isolates and human lung carcinoma cells.

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Conflict of interest statement

The authors declare that no competing interest exists with this manuscript.

Figures

Figure 1
Figure 1
TEM analysis of AgNPs.
Figure 2
Figure 2
Synthesis of silver nanoparticles: (a) control (silver nitrate), (b) silver nanoparticles (TNP), and (a) aqueous leaf extract (T).
Figure 3
Figure 3
Influence of different parameters on TNP synthesis: (a) effect of temperature, (b) effect of pH, (c) effect of substrate concentration, and (d) effect of the ratio of the plant extract.
Figure 4
Figure 4
Characterization of TNP: (a) UV-visible spectra, (b) TEM analysis, (c) zeta potential analysis, and (d) XRD of TNP.
Figure 5
Figure 5
FTIR analysis: (a) FTIR of the leaf aqueous extract and (b) FTIR of TNP of T. procumbens.
Figure 6
Figure 6
Cell cytotoxicity. AgNP: chemically synthesized silver nanoparticles; T: leaf aqueous extract; TNP: biogenic silver nanoparticles (the error bars shown on each histogram indicate standard deviation).
Figure 7
Figure 7
HRLC-MS: (a) HRLC-MS of TNP synthesized by HPLC fraction (TNP11) and (b) HRLC-MS of TNP synthesized by the whole aqueous leaf extract.
See this image and copyright information in PMC

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