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. 2022 Sep;49(9):8827-8834.
doi: 10.1007/s11033-022-07732-3. Epub 2022 Jul 7.

Genotyping Helicobacter pylori and fgf7 gene expression in gastric cancer

Manouchehr Ahmadi Hedayati  1   2 , Delniya Khani  3   4 , Hamed Bashiri  5
Affiliations

Genotyping Helicobacter pylori and fgf7 gene expression in gastric cancer

Manouchehr Ahmadi Hedayati et al. Mol Biol Rep. 2022 Sep.

Abstract

Background: Helicobacter pylori as the causative agent of the most common chronic bacterial infectious disease in human still involves a range of clinical challenging complications. In this meantime, the survey of the interaction between H. pylori virulence genes expression and its consequences on gastric antral epithelial cells is Controversial. This study surveyed the correlations between H. pylori cag Pathogenicity Island and virulence factors genes with Fgf7 gene expression as an angiogenic factor in developing gastric cancer in gastric antral epithelial cells of patients with H. pylori infection.

Method: Gastric antral biopsy samples collected from patients out of exclusion criteria, including consumption of tobacco, alchohol and anti-H. pylori drugs, were categorized into gastric cancer (case group n:53) and gastritis (control group n:50) with and without H. pylori infection to detect changes in cDNA of fgf7 in gastric antral epithelial cells by using Real Time RT PCR. Extracted total RNA from gastric antral biopsy samples was used to synthesize cDNA for real time PCR. Furthermore, the cDNA of H. pylori cag Pathogenicity Island and other virulence factors genes were detected by using specific designed primers and simple PCR.

Results: Fgf7 gene expression revealed a significantly increase in gastric antral epithelial cells of gastric cancer and H. pylori-positive patients in contrast with gastritis and H. pylori-negative patients (p < 0.05). In the meanwhile, cag Pathogenicity Island and hopQ genotypes showed a positive correlation with Fgf7 gene expression (fold changes of cDNA) in gastric antral epithelial cells (p < 0.05).

Conclusion: This study revealed an obvious correlation between Fgf7 gene expression in gastric antral epithelial cells of patients with H. pylori carcinogenic genotypes infection and some host factors including age.

Keywords: Cag Pathogenicity Island; Fibroblast growth factor 7 gene; Gastric cancer; Helicobacter pylori.

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