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Review
. 2022 May;11(5):1664-1671.
doi: 10.4103/jfmpc.jfmpc_1479_21. Epub 2022 May 14.

Laboratory diagnosis of mucormycosis: Present perspective

Munesh K Gupta  1 , Nilesh Kumar  2 , Neeraj Dhameja  3 , Arti Sharma  1 , Ragini Tilak  1
Affiliations
Review

Laboratory diagnosis of mucormycosis: Present perspective

Munesh K Gupta et al. J Family Med Prim Care. 2022 May.

Abstract

Upsurge in mucormycosis cases in the second wave of SARS CoV2 infection in India has been reported. Uncontrolled diabetes is the major predisposing risk factor for these cases. The early diagnosis and surgical intervention with medical treatment may result in good clinical outcomes. The glycaemic control in diabetic patients also favours better treatment outcome in patients suffering from mucormycosis.

Keywords: Diabetes; GMS; KOH wet mount; RSA protein; Rhizopus arrhizus; steroid.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Black discolouration of the left cheek with left eye, left lip and left nasal cavity
Figure 2
Figure 2
CT axial images of skull showing ill defined soft tissue density noted filling right maxillary, ethmoid and sphenoid sinus with rarefaction of anterior and medial wall of maxillary sinus with mild right orbital bulge and periorbital swelling
Figure 3
Figure 3
Excised tissue sample for microbiological investigation
Figure 4
Figure 4
KOH wet mount of the excised tissue showing broad aseptate hyaline hyphae with right angle branching (Arrow), 400×
Figure 5
Figure 5
Cottony-fluffy growth of Rhizopus arrhizus on SDA agar
Figure 6
Figure 6
LPCB wet mount showing Rhizoid (a), sporangiophore (b), Apophysis (c), columella (d), collarets (e), sporangium (f), 400×
Figure 7
Figure 7
H and E staining of the excised tissue showing the necrosis (a) with plenty of broad aseptate hyphae (arrow), 400×
Figure 8
Figure 8
Pink coloured aseptate fungal hyphae (arrow), 400×
Figure 9
Figure 9
Dark brown-black coloured aspetate fungal hyphae (arrow), 400×
See this image and copyright information in PMC

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