Thulium Fiber Laser's Dust for Stone Composition Analysis: Is It Enough? A Pilot Study

Abstract

Introduction: We aimed to evaluate if the biochemical composition of urinary stones can be determined by analyzing the stone dust only, and whether a photo taken during the surgery could be useful for completing the morpho-constitutional analysis. Materials and Methods: Twenty patients went through a retrograde intrarenal surgery for renal stone treatment with thulium fiber laser (Fiber Dust; Quanta, 2020) using 150 μm silica core laser fibers. After laser lithotripsy, residual fragments (RF) were removed with a basket (ZeroTip; Boston Scientific) and spontaneously floating stones particles were considered stone dust and were aspirated through the working channel. Pairs of RF and stone dust were labeled and sent to analysis by scanning electron microscopy and Fourier transform infrared spectroscopy. Photos of the stone (surface and section) were taken from videos recorded during the surgery. Results: A total of 20 patients were included in this study. Mean age was 49.8 years with metabolic and genetic disorders. Mean stone volume was 750 mm³ for ureteral stones and 2334 mm³ for renal stones. Mean stone density was 1187 HU. Positive urine culture was found in 25% patients. In 2/20 (10%), the biochemistry differed only in the relative proportions of each constituent, while in 5/20 (25%), only one component was missing. Laser crystalline conversion was found in 3/20 (15%). Whewellite and weddellite layers were found in photos, thus adding missing information from dust stone analysis. Conclusion: By analyzing aspirated dust through the ureteroscope's working channel with physical techniques, we can understand the lithogenic process of the urinary stone, without needing to analyze the stone fragment. Morphologic analysis, given by a proper stone picture, adds missing information in specific cases.

Keywords: Fourier transform infrared spectroscopy; lithotripsy; renal calculus biochemistry; stone analysis; stone dust; stone picture; thulium fiber laser; urinary stones; urolithiasis.