Correction: Nguyen et al. The Nephrotoxin Puromycin Aminonucleoside Induces Injury in Kidney Organoids Differentiated from Induced Pluripotent Stem Cells. Cells 2022, 11, 635

Abstract

In the original publication [...].

Figure 1

Lobular kidney organoids contain distinct kidney structures. (a) Schematic depiction of the protocol for generating kidney organoids. (b) Overview of iPSC spheroids at D8 after generation (a.,b.) and kidney organoids UMK2 at D21 (c.,d.) with binocular. (a.) 4× magnification under light microscope. Scale bar depicts 500 µm. (b.) 10× magnification under light microscope. Scale bar depicts 200 µm. (c.) 1× magnification. Scale bar depicts 2000 µm. (d.) 4× magnification. Scale bar depicts 2000 µm. (c) Morphology of organoid section via histological H&E staining. A typical glomerulus-like structure is depicted by a dashed rectangle. Tubule-like structures are marked with an asterisk. Scale bar depicts 50 µm. (d) Confocal pictures of glomerular (ACTN4, yellow) and tubular (LTL, green) structures in UMK1 sections. Nuclear OCT2 (POU2F2) (red) is expressed in UMK1 sections. A glomerulus-like structure was marked with an arrow. Scale bar depicts 50 µm. (e) Monitoring of iPSC spheroids and kidney organoids in a dextran uptake assay. (a.) iPSC spheroids treated with dextran after 4 h pulse. (b.) kidney organoids treated with dextran after 4 h pulse. (c.) kidney organoids treated with dextran after 24 h chase. Scale bar depicts 200 µm.