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. 2022 Jun 25;23(13):7062.
doi: 10.3390/ijms23137062.

Microsatellite Instability in Russian Patients with Colorectal Cancer

Vitaly Shubin  1 , Yury Shelygin  1 , Sergey Achkasov  1 , Oleg Sushkov  1 , Ilya Nazarov  1 , Alexey Ponomarenko  1 , Iuliia Alimova  1 , Anna Loginova  1 , Aleksey Tsukanov  1
Affiliations

Microsatellite Instability in Russian Patients with Colorectal Cancer

Vitaly Shubin et al. Int J Mol Sci. .

Abstract

The aim of this study was to determine the characteristics of Russian patients with microsatellite instability (MSI) tumors. MSI in the tumor was determined in 514 patients with colon cancer using PCR and subsequent fragment analysis for five markers (NR21, NR24, BAT25, BAT26, and NR27). In the presence of microsatellite instability, the mismatch repair (MMR) system genes were examined using the NGS and MLPA methods to establish the diagnosis of Lynch syndrome. The overall frequency of MSI tumors was 15%: at stage I—19% (9/48), at stage II—21% (44/213), at stage III—16% (26/160), and at stage IV—2% (2/93). Patients with MSI tumors differed in the age of diagnosis, tumor localization, time of cancer recurrence, and stage of the disease. The overall and disease-free survival of patients whose tumors had MSI status was higher than that of patients with microsatellite-stable status, p = 0.04 and p = 0.02, respectively. Analysis of overall and disease-free survival of patients with Lynch syndrome and patients with sporadic colon cancer, but with MSI status, did not reveal significant differences, p = 0.52 and p = 0.24, respectively. The age of patients with Lynch syndrome was significantly younger than that of patients with sporadic colon cancer whose tumors had MSI status (p < 0.001).

Keywords: Lynch syndrome; colorectal cancer; microsatellite instability.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Overall survival of patients with CRC (stages I–IV) depending on the status of microsatellite instability.
Figure 2
Figure 2
Disease-free survival of patients with CRC (stages I–IV) depending on the status of microsatellite instability.
Figure 3
Figure 3
Rates of overall and disease-free survival in patients with Lynch syndrome and patients with MSI status of sporadic tumor: (A) overall survival; (B) disease-free survival.
Figure 4
Figure 4
MSI frequencies at stages I–IV CRC.
Figure 5
Figure 5
Age of patients whose tumors were MSS, MSI with Lynch’s syndrome, and MSI in sporadic cancer.
Figure 6
Figure 6
Frequency of MSI in the right colon, left colon, and rectum in Germany, Russia, and Italy.
Figure 7
Figure 7
Overall survival of patients with CRC (stages I–III) depending on the status of microsatellite instability.
See this image and copyright information in PMC

References

    1. Tsukanov A.S., Shelygin Y.A., Shubin V.P. Microsatellite instability in colorectal cancer (review) Koloproktologia. 2017;2:100–104. doi: 10.33878/2073-7556-2017-0-2-100-104. - DOI
    1. Boland C.R., Goel A. Microsatellite Instability in Colorectal Cancer. Gastroenterology. 2010;138:2073–2087.e3. doi: 10.1053/j.gastro.2009.12.064. - DOI - PMC - PubMed
    1. Luchini C., Bibeau F., Ligtenberg M., Singh N., Nottegar A., Bosse T., Miller R., Riaz N., Douillard J.-Y., Andre F., et al. ESMO recommendations on microsatellite instability testing for immunotherapy in cancer, and its relationship with PD-1/PD-L1 expression and tumour mutational burden: A systematic review-based approach. Ann. Oncol. 2019;30:1232–1243. doi: 10.1093/annonc/mdz116. - DOI - PubMed
    1. Goel A., Nagasaka T., Hamelin R., Boland C.R. An Optimized Pentaplex PCR for Detecting DNA Mismatch Repair-Deficient Colorectal Cancers. PLoS ONE. 2010;5:e9393. doi: 10.1371/annotation/572bb6d3-0315-40b1-a6d7-ce818809b5ea. - DOI - PMC - PubMed
    1. Sargent D.J., Marsoni S., Monges G., Thibodeau S.N., Labianca R., Hamilton S.R., French A.J., Kabat B., Foster N.R., Torri V., et al. Defective Mismatch Repair As a Predictive Marker for Lack of Efficacy of Fluorouracil-Based Adjuvant Therapy in Colon Cancer. J. Clin. Oncol. 2010;28:3219–3226. doi: 10.1200/JCO.2009.27.1825. - DOI - PMC - PubMed

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