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. 2022 Jun 23;11(13):1666.
doi: 10.3390/plants11131666.

Bulgarian Medicinal Extracts as Natural Inhibitors with Antiviral and Antibacterial Activity

Ivanka Nikolova  1 , Tsvetelina Paunova-Krasteva  2 , Zdravka Petrova  1   3 , Petar Grozdanov  4 , Nadya Nikolova  1 , Georgi Tsonev  1   5 , Alexandros Triantafyllidis  1   5 , Stoyan Andreev  1   5 , Madlena Trepechova  1 , Viktoria Milkova  6 , Neli Vilhelmova-Ilieva  1
Affiliations

Bulgarian Medicinal Extracts as Natural Inhibitors with Antiviral and Antibacterial Activity

Ivanka Nikolova et al. Plants (Basel). .

Abstract

Background: Bulgaria is a country with a wide range of medicinal plants, with uses in traditional medicine dating back for centuries.

Methods: Disc diffusion assay was used to evaluate the antimicrobial activity of the plant extracts. A cytopathic effect inhibition test was used for the assessment of the antiviral activity of the extracts. The virucidal activity of the extracts, their influence on the stage of viral adsorption, and their protective effect on uninfected cells were reported using the end-point dilution method, and Δlgs was determined as compared to the untreated controls.

Results: The results of the study reveal that the antibacterial potential of G. glabra and H. perforatum extracts in Gram-positive bacteria is more effective than in Gram-negative bacteria. When applied during the replication of HSV-1 and HCov-OC-43, only some of the extracts showed weak activity, with SI between 2 to 8.5. Almost all tested extracts inhibited the extracellular virions of the studied enveloped viruses (HSV-1 and HCov-OC-43) to a greater extent than of the non-enveloped viruses (PV-1 and HAdV-5). They inhibited the stage of viral adsorption (HSV-1) in the host cell (MDBK) to varying degrees and showed a protective effect on healthy cells (MDBK) before they were subjected to viral invasion (HSV-1).

Conclusion: The antipathogenic potential of extracts of H. perforatum and G. glabra suggests their effectiveness as antimicrobial agents. All 13 extracts of the Bulgarian medicinal plants studied can be used to reduce viral yield in a wide range of viral infections.

Keywords: antimicrobial activity; herpes simplex virus-1; human adenovirus-5; human coronavirus; natural extracts; poliovirus-1; viral adsorption; virucidal activity.

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Conflict of interest statement

The authors declare no conflict of interest. The sponsors had no role in the design, execution, interpretation, or writing of the study.

Figures

Figure 1
Figure 1
Determination of the anti-microbial activity of theextracts from G. glabra (A,C) and H. perforatum (B,D) against Bacillus subtilis 168 (A,B) and Staphylococcus aureus 29,213 (C,D). The results were obtained by the application of 40 mg/mL water–ethanol extracts using the disc diffusion method. Positive control: Gentamicin discs–15 µg/mL, (B,D). All experiments were repeated three times, and similar results were obtained.
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References

    1. Piret J., Boivin G. Resistance of herpes simplex viruses to nucleoside analogues: Mechanisms, prevalence, and management. Antimicrob. Agents Chemother. 2011;55:459–472. doi: 10.1128/AAC.00615-10. - DOI - PMC - PubMed
    1. Stoitsova S., Paunova-Krasteva T., Borisova D. Modulation of Biofilm Growth by Sub-Inhibitory Amounts of Antibacterial Substances. In: Dhanasekaran D., Thajuddin N., editors. Microbial Biofilms—Importance and Applications. IntechOpen; London, UK: 2016. pp. 441–462.
    1. Borges A., Abreu A.C., Dias C., Saavedra M.J., Borges F., Simões M. New perspectives on the use of phytochemicals as an emergent strategy to control bacterial infections including biofilms. Molecules. 2016;21:877. doi: 10.3390/molecules21070877. - DOI - PMC - PubMed
    1. Othman L., Sleiman A., Abdel-Massih R.M. Antimicrobial activity of polyphenols and alkaloids in middle eastern plants. Front. Microbiol. 2019;10:911. doi: 10.3389/fmicb.2019.00911. - DOI - PMC - PubMed
    1. Poli J.P., Guinoiseau E., De Rocca Serra D., Sutour S., Paoli M., Tomi F., Quilichini Y., Berti L., Lorenzi V. Anti-quorum sensing activity of 12 essential oils on chromobacterium violaceum and specific action of cis-cis-p-menthenolide from corsican Mentha suaveolens ssp. Insularis. Molecules. 2018;23:2125. doi: 10.3390/molecules23092125. - DOI - PMC - PubMed

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