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. 2022 Jul 4:17:2907-2924.
doi: 10.2147/IJN.S360000. eCollection 2022.

Beneficial Effect of Selenium Doped Carbon Quantum Dots Supplementation on the in vitro Development Competence of Ovine Oocytes

Mengqi Wang #  1 , Jingyu Ren #  1 , Zhanpeng Liu  2 , Shubin Li  3 , Liya Su  1 , Biao Wang  4 , Daoning Han  1 , Gang Liu  1
Affiliations

Beneficial Effect of Selenium Doped Carbon Quantum Dots Supplementation on the in vitro Development Competence of Ovine Oocytes

Mengqi Wang et al. Int J Nanomedicine. .

Abstract

Background: After the synthesis of selenium doped carbon quantum dots (Se/CDs) via a step-by-step hydrothermal synthesis method with diphenyl diselenide (DPDSe) as precursor, the beneficial effects of Se/CDs' supplementation on the in vitro development competence of ovine oocytes were firstly investigated in this study by the assay of maturation rate, cortical granules' (CGs) dynamics, mitochondrial activity, reactive oxygen species (ROS) production, epigenetic modification, transcript profile, and embryonic development competence.

Results: The results showed that the Se/CDs' supplementation during the in vitro maturation (IVM) process not only enhanced the maturation rate, CGs' dynamics, mitochondrial activity and embryonic developmental competence of ovine oocytes, but remarkably decreased the ROS production level of ovine oocytes. In addition, the expression levels of H3K9me3 and H3K27me3 in the ovine oocytes were significantly up-regulated after the Se/CDs' supplementation, in consistent with the expression levels of 5mC and 5hmC. Moreover, 2994 up-regulated differentially expressed genes (DEGs) and 846 repressed DEGs were found in the oocytes after the Se/CDs' supplementation. According to the analyses of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), these DEGs induced by the Se/CDs' supplementation were positively related to the progesterone mediated oocyte maturation and mitochondrial functions. And these remarkably up-regulated expression levels of DEGs related to oocyte maturation, mitochondrial function, and epigenetic modification induced by the Se/CDs' supplementation further confirmed the beneficial effect of Se/CDs' supplementation on the in vitro development competence of ovine oocytes.

Conclusion: The Se/CDs prepared in our study significantly promoted the in vitro development competence of ovine oocytes, benefiting the extended research about the potential applications of Se/CDs in mammalian breeding technologies.

Keywords: Se/CDs; mitochondrial activity; ovine oocytes and transcript profiles; reactive oxygen species production.

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Conflict of interest statement

The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
The characteristic f Se/CDs. (A) The TEM characteristic of Se/CDs. (B) The 200 nm, 10 nm morphology and lattice of Se/CDs. (C) XPS spectrum of Se-CDs. (D) The FT-IR spectrum scanning from 4000 cm-1 to 800 cm-1. (E) The UV absorption (blue), excitation (black), and emission (red) spectra of Se-CDs (λex =340 nm, λem = 360 nm). (F) The normalized excitation-dependent fluorescence emissions of Se/CDs (340–480 nm).
Figure 2
Figure 2
The effect of Se/CDs’ supplementation on the IVM efficiency of ovine oocytes. (A) Morphology of COCs before IVM, COCs after IVM and MII oocytes. (B) Maturation rates.
Figure 3
Figure 3
The effect of Se/CDs’ supplementation on the cytoplasmic maturation, mitochondrial activities and ROS production levels of ovine MII oocytes.
Figure 4
Figure 4
The effect of Se/CDs’ supplementation on the histone methylation levels of ovine MII oocytes. (A) H3K9me3 immunofluorescence staining of ovine MII oocytes. (B) H3K27me3 immunofluorescence staining of ovine MII oocytes. (C) Relative fluorescence intensity of H3K9me3. (D) Relative fluorescence intensity of H3K27me3.
Figure 5
Figure 5
The effect of Se/CDs’ supplementation on the DNA methylation levels of ovine MII oocytes. (A) 5mC immunofluorescence staining of ovine MII oocytes. (B) 5hmC immunofluorescence staining of ovine MII oocytes. (C) Relative fluorescence intensity of 5mC. (D) Relative fluorescence intensity of 5hmC.
Figure 6
Figure 6
The effect of Se/CDs’ supplementation on the embryonic development potentials of ovine MII oocytes. (A) Morphology of 2-cells after IVF. (B) Fertilization rates. (C) Blastocyst development rates.
Figure 7
Figure 7
The effect of Se/CDs’ supplementation on the transcript profiles of ovine MII oocytes. (A) Numbers of differentially expressed genes (DEGs) that are abnormally repressed (blue) or upregulated (red) in the Se/CDs group compared to the NC group. (B) Heatmap illustration showing the DEGs in the Se/CDs group compared to the NC group. (C) Top 20 KEGG pathways enriched in the Se/CDs group compared to the NC group. (D) GO analyses of the DEGs in the Se/CDs group compared to the NC group. (E) Expression levels of DEGs related to oocyte maturation, mitochondrial functions and epigenetic modifications in the NC and Se/CDs groups.
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