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. 2022 Jun 23:13:891902.
doi: 10.3389/fmicb.2022.891902. eCollection 2022.

IS 6110 Copy Number in Multi-Host Mycobacterium bovis Strains Circulating in Bovine Tuberculosis Endemic French Regions

Ciriac Charles  1   2 , Cyril Conde  2 , Franck Biet  2 , Maria Laura Boschiroli  1 , Lorraine Michelet  1
Affiliations

IS 6110 Copy Number in Multi-Host Mycobacterium bovis Strains Circulating in Bovine Tuberculosis Endemic French Regions

Ciriac Charles et al. Front Microbiol. .

Abstract

IS6110 is an insertion sequence found in the Mycobacterium tuberculosis complex, to which Mycobacterium bovis belongs, which can play a role in genome plasticity and in bacterial evolution. In this study, the abundance and location of IS6110 on M. bovis genomic data of French animal field strains were studied. A first analysis was performed on a panel of 81 strains that reflect the national M. bovis population's genetic diversity. The results show that more than one-third of them are IS6110 multicopy and that 10% have IS6110 in a high copy number (more than 6 copies). Multicopy strains are those circulating in the regions where prevalence was above the national average. Further study of 93 such strains, with an IS6110 copy number of 10-12, showed stability of IS6110 copy number and genome location over time and between host species. The correlation between M. bovis multicopy strains and high bovine tuberculosis (bTB) prevalence leads us to consider whether their epidemiological success could be partly due to genetic changes originated by IS6110 transposition.

Keywords: France; IS6110; Mycobacterium bovis; bovine tuberculosis (bTB); insertion sequence (IS).

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
IS6110 occurrence in Mycobacterium bovis French genetic diversity. Heatmap showing the presence and genomic position of IS6110 in 81 genomes (80 genomes of Mycobacterium bovis representing French diversity and Mb3601 reference strains). The tree is based on 8,981 whole-genome single nucleotide polymorphism. The strains are grouped in 7 clusters that have been previously defined (Hauer et al., 2019).
FIGURE 2
FIGURE 2
IS6110 in M. bovis SB0120-CO over time and between different host species. Heatmap showing the presence or absence of IS6110 in 93 sympatric SB0120-CO genomes. Black spots show the presence of IS6110 in a specific site. Nucleotide positions of each IS6110 site are described in Supplementary Table 3. The tree is based on 124 whole-genome single nucleotide polymorphism.
FIGURE 3
FIGURE 3
Gene-environment of IS6110 sites in M. bovis French diversity. Gene Ontology (GO) of coding sequences (CDSs) near IS6110 sites in 3 M. bovis groups (SB0120-DHV in green, SB0120-CO in black, and Cluster A/F4 family in pink).
See this image and copyright information in PMC

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