Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jan 31;18(1):1-13.
doi: 10.6026/97320630018001. eCollection 2022.

Insights from the analysis of draft genome sequence of Crocus sativus L

Sheetal Ambardar  1 , Jyoti Vakhlu  2 , Ramanathan Sowdhamini  1   3
Affiliations

Insights from the analysis of draft genome sequence of Crocus sativus L

Sheetal Ambardar et al. Bioinformation. .

Abstract

Saffron (Crocus sativus L.) is the low yielding plant of medicinal and economic importance. Therefore, it is of interest to report the draft genome sequence of C. sativus. The draft genome of C. sativus has been assembled using Illumina sequencing and is 3.01 Gb long covering 84.24% of genome. C. sativus genome annotation identified 53,546 functional genes (including 5726 transcription factors), 862,275 repeats and 964,231 SSR markers. The genes involved in the apocarotenoids biosynthesis pathway (crocin, crocetin, picrocrocin, and safranal) were found in the draft genome analysis.

Keywords: Crocus sativus; MYB TFs; Orthology analysis; SSR markers; apocarotene biosynthesis pathway; de-novo genome assembly.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of Interest.

Figures

Figure 1
Figure 1
Schematic of de-novo genome assembly and annotation pipeline. Black colour text represents the analytical processes and Red colour text represents the software/instrument used to perform the processes.
Figure 2
Figure 2
Crocus sativus unigenes mapping to top 15 plant species wherein most of the proteins annotated against Asparagus officinalis.
Figure 3
Figure 3
Orthology analysis of Crocus sativus with neighboring plants from same order (Asparagus officinalis, Phalaenopsis equestris, Apostatia shenzhenica) along with Oryza sativa representing 7328 proteins clusters to be conserved in all the five plant species, whereas 2510 proteins cluster were unique to C. sativus only.
See this image and copyright information in PMC

References

    1. Choi JY, et al. Genome Biol. . 2020;21:21. - PMC - PubMed
    1. Liu J, et al. Genome Biol. . 2020;21:121. - PMC - PubMed
    2. {'text': '', 'ref_index': 2, 'ids': [{'type': 'DOI', 'value': '10.1186/s13059-020-02029-9', 'is_inner': False, 'url': 'https://doi.org/10.1186/s13059-020-02029-9'}]}
    1. Harkess A, et al. Nat Commun. . 2017;8:1279. - PMC - PubMed
    1. Alonge M, et al. Genetics. . 2020;216:599. - PMC - PubMed
    1. Xia E, et al. Mol Plant. 2020;13:1013. - PubMed

LinkOut - more resources