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. 2022 Nov;49(11):1185-1191.
doi: 10.1111/jcpe.13697. Epub 2022 Jul 26.

Toll-like receptor-1, -2, and -6 genotypes in relation to salivary human beta-defensin-1, -2, -3 and human neutrophilic peptide-1

Mervi Gürsoy  1   2 , Eija Könönen  1 , Qiushui He  3   4 , Anna Liukkonen  1 , Sisko Huumonen  5   6 , Ulvi Kahraman Gürsoy  1
Affiliations

Toll-like receptor-1, -2, and -6 genotypes in relation to salivary human beta-defensin-1, -2, -3 and human neutrophilic peptide-1

Mervi Gürsoy et al. J Clin Periodontol. 2022 Nov.

Abstract

Aim: To examine whether functional gene polymorphisms of toll-like receptor (TLR)1, TLR2, and TLR6 are related to the salivary concentrations of human beta-defensins (hBDs)-1, -2, -3, and human neutrophilic peptide (HNP)-1.

Materials and methods: Polymorphisms of TLR1 (rs5743618), TLR2 (rs5743708), and TLR6 (rs5743810) were genotyped by PCR-based pyrosequencing from the salivary samples of 230 adults. Salivary hBD-1, -2, -3, and HNP-1 concentrations were measured using enzyme-linked immunosorbent assay. General and periodontal health examinations, including panoramic radiography, were available for all participants.

Results: The genotype frequencies for wild types and variant types were as follows: 66.5% and 33.5% for TLR1, 95.5% and 4.5% for TLR2, and 25.1% and 74.9% for TLR6, respectively. The TLR2 heterozygote variant group exhibited higher salivary hBD-2 concentrations than the TLR2 wild-type group (p = .038). On the contrary, elevated hBD-2 concentrations were detected in the TLR6 wild-type group compared with the TLR6 heterozygote and homozygote variant group (p = .028). The associations between TLR6 genotypes and salivary hBD-2 concentrations remained significant after adjusting them for periodontal status, age, and smoking.

Conclusion: hBD-2 concentrations in saliva are related to TLR2 and TLR6 polymorphisms, but only the TLR6 genotype seems to exhibit an independent association with the salivary hBD-2 concentrations.

Keywords: defensins; genetic; periodontium; polymorphism; saliva.

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Conflict of interest statement

The authors declare no conflict of interest.

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References

    1. Barreiro, L. B. , Ben‐Ali, M. , Quach, H. , Laval, G. , Patin, E. , Pickrell, J. K. , Bouchier, C. , Tichit, M. , Neyrolles, O. , Gicquel, B. , Kidd, J. R. , Kidd, K. K. , Alcaïs, A. , Ragimbeau, J. , Pellegrini, S. , Abel, L. , Casanova, J. L. , & Quintana‐Murci, L. (2009). Evolutionary dynamics of human Toll‐like receptors and their different contributions to host defense. PLoS Genetics, 5, e1000562. 10.1371/journal.pgen.1000562 - DOI - PMC - PubMed
    1. Bhattacharyya, S. , Ghosh, S. K. , Shokeen, B. , Eapan, B. , Lux, R. , Kiselar, J. , Nithianantham, S. , Young, A. , Pandiyan, P. , McCormick, T. S. , & Weinberg, A. (2016). FAD‐I, a Fusobacterium nucleatum cell wall‐associated diacylated lipoprotein that mediates human beta defensin 2 induction through Toll‐like receptor‐1/2 (TLR‐1/2) and TLR‐2/6. Infection and Immunity, 84, 1446–1456. 10.1128/IAI.01311-15 - DOI - PMC - PubMed
    1. Chang, A. M. , Kantrong, N. , & Darveau, R. P. (2021). Maintaining homeostatic control of periodontal epithelial tissue. Periodontology 2000, 86, 188–200. 10.1111/prd.12369 - DOI - PubMed
    1. Corr, S. C. , & O'Neill, L. A. (2009). Genetic variation in Toll‐like receptor signalling and the risk of inflammatory and immune diseases. Journal of Innate Immunity, 1, 350–357. 10.1159/000200774 - DOI - PubMed
    1. Dale, B. A. , & Fredericks, L. P. (2005). Antimicrobial peptides in the oral environment: Expression and function in health and disease. Current Issues in Molecular Biology, 7, 119–133. 10.1093/jac/dki103 - DOI - PMC - PubMed

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