Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2536:3-11.
doi: 10.1007/978-1-0716-2517-0_1.

Obtaining and Maintaining Cultures of Pinewood Nematodes Bursaphelenchus xylophilus from Wild Dauers

Carla S Pimentel  1 , Paulo N Firmino  2 , Matthew P Ayres  3
Affiliations

Obtaining and Maintaining Cultures of Pinewood Nematodes Bursaphelenchus xylophilus from Wild Dauers

Carla S Pimentel et al. Methods Mol Biol. 2022.

Abstract

The establishment of laboratory isolates of the pinewood nematode Bursaphelenchus xylophilus, the causal agent of the pine wilt disease, has been crucial to research on this important forest pathogen. Here we describe a simple, low-cost, and easy way to obtain samples of wild populations of B. xylophilus by culturing dauers extracted directly from the insect vector.

Keywords: Botrytis cinerea; Forest pathogen; Malt extract agar; Pine forests; Pinus; Wood fiber.

PubMed Disclaimer

References

    1. Giblin-Davis RM, Davies KA, Morris K et al (2003) Evolution of parasitism in insect-transmitted plant nematodes. J Nematol 35:133–141 - PubMed - PMC
    1. Ma HB, Lu Q, Liang J et al (2011) Functional analysis of the cellulose gene of the pine wood nematode, Bursaphelenchus xylophilus, using RNA interference. Genet Mol Res 10903:1931–1941 - DOI
    1. Mori Y, Miyahara F, Tsutsumi Y et al (2008) Relationship between resistance to pine wilt disease and the migration and proliferation of pine wood nematodes. Eur J Plant Pathol 1224:529–538 - DOI
    1. Kuroda K (2004) Inhibiting factors of symptom development in several Japanese red pine (Pinus densiflora) families selected as resistant to pine wilt. J For Res 9:217–224 - DOI
    1. Pimentel CS, Firmino PN, Calvão T et al (2017) Pinewood nematode population growth in relation to pine phloem chemical composition. Plant Pathol 665:856–864 - DOI

Publication types

LinkOut - more resources