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. 2022 Dec;194(12):5748-5766.
doi: 10.1007/s12010-022-04061-w. Epub 2022 Jul 11.

Native SodB Overexpression of Synechocystis sp. PCC 6803 Improves Cell Growth Under Alcohol Stresses Whereas Its Gpx2 Overexpression Impacts on Growth Recovery from Alcohol Stressors

Phuwanet Vachiranuvathin  1 , Vetaka Tharasirivat  1 , Thitaporn Hemnusornnanon  1 , Saowarath Jantaro  2
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Native SodB Overexpression of Synechocystis sp. PCC 6803 Improves Cell Growth Under Alcohol Stresses Whereas Its Gpx2 Overexpression Impacts on Growth Recovery from Alcohol Stressors

Phuwanet Vachiranuvathin et al. Appl Biochem Biotechnol. 2022 Dec.

Abstract

To overcome the limited resistance to alcohol stress, genetically engineered Synechocystis sp. PCC 6803 strains with overexpressions of genes related with the ROS detoxification system (sodB and gpx2, which encode superoxide dismutase and glutathione peroxidase, respectively) were developed. Three engineered strains including a sodB-overexpressing strain (OE + S), a gpx2-overexpressing strain (OE + G), and a sodB/gpx2-overexpressing strain (OE + SG) grew similarly as wild-type control under normal condition. When compared to wild-type control, OE + S and OE + SG strains grew faster for 4 days under 2.0% (v/v) ethanol and 0.3% (v/v) n-butanol conditions, as well as having higher chlorophyll a levels. On the other hand, the prominent growth recovery of OE + G and OE + SG was noted within 4 days in normal BG11 medium after treating cells with high alcohol stresses for 1 h, in particular 15% ethanol and 2.5% n-butanol. Under 4 days of recovery from butanol stress, specific levels of intracellular pigments including chlorophyll a and carotenoids were dramatically increased in all modified strains. The overexpression of antioxidant genes then revealed a significant improvement of alcohol tolerance in Synechocystis sp. PCC 6803.

Keywords: Alcohol tolerance; Glutathione peroxidase; Growth recovery; Superoxide dismutase; Synechocystis sp. PCC 6803.

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References

    1. Shima, J., & Takagi, H. (2009). Stress-tolerance of baker’s-yeast (Saccharomyces cerevisiae) cells: Stress-protective molecules and genes involved in stress tolerance. Biotechnology and Applied Biochemistry, 53, 155–164. - DOI - PubMed
    1. Abe, H., Fujita, Y., Takaoka, Y., Kurita, E., Yano, S., Tanaka, N., & Nakayama, K. (2009). Ethanol-tolerant Saccharomyces cerevisiae strains isolated under selective conditions by over-expression of a proofreading-deficient DNA polymerase δ. Journal of Bioscience and Bioengineering, 108, 199–204. - DOI - PubMed
    1. Kim, H. S., Kim, N. R., Yang, J., & Choi, W. (2011). Identification of novel genes responsible for ethanol and/or thermotolerance by transposon mutagenesis in Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 91, 1159–1172. - DOI - PubMed
    1. Aono, R. (1998). Improvement of organic solvent tolerance level of Escherichia coli by overexpression of stress-responsive genes. Extremophiles, 2, 239–248. - DOI - PubMed
    1. Kobayashi, K., Tsukagoshi, N., & Aono, R. (2001). Suppression of hypersensitivity of Escherichia coli acrB mutant to organic solvents by integrational activation of the acrEF operon with the IS1 of IS2 element. Journal of Bacteriology, 183, 2646–2653. - DOI - PubMed - PMC

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