Fluorescent/magnetic nano-aggregation via electrostatic force between modified quantum dot and iron oxide nanoparticles for bimodal imaging of U87MG tumor cells

Minchuan Luo¹, Hiroshi Yukawa^{2

3

4

5

6}, Yoshinobu Baba^{7

8

9}

Affiliations

¹ Nanobio Analytical Chemistry, Biomolecular Chemistry, Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.
² Nanobio Analytical Chemistry, Biomolecular Chemistry, Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. h.yukawa@nanobio.nagoya-u.ac.jp.
³ Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. h.yukawa@nanobio.nagoya-u.ac.jp.
⁴ Institute of Quantum Life Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, Anagawa, Inageku, Chiba, 263-8555, Japan. h.yukawa@nanobio.nagoya-u.ac.jp.
⁵ Advanced Analytical and Diagnostic Imaging Center (AADIC)/Medical Engineering Unit (MEU), Nagoya University Institute for Advanced Research, B3 Unit, Tsurumai 65, Showa-ku, Nagoya, 466-8550, Japan. h.yukawa@nanobio.nagoya-u.ac.jp.
⁶ Development of Quantum-Nano Cancer Photoimmunotherapy for Clinical Application of Refractory Cancer, Nagoya University, Tsurumai 65, Showa-ku, Nagoya, 466-8550, Japan. h.yukawa@nanobio.nagoya-u.ac.jp.
⁷ Nanobio Analytical Chemistry, Biomolecular Chemistry, Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. babaymtt@chembio.nagoya-u.ac.jp.
⁸ Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. babaymtt@chembio.nagoya-u.ac.jp.
⁹ Institute of Quantum Life Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, Anagawa, Inageku, Chiba, 263-8555, Japan. babaymtt@chembio.nagoya-u.ac.jp.

PMID: 35819752
DOI: 10.1007/s44211-022-00153-z

Fluorescent/magnetic nano-aggregation via electrostatic force between modified quantum dot and iron oxide nanoparticles for bimodal imaging of U87MG tumor cells

Minchuan Luo et al. Anal Sci. 2022 Sep.

. 2022 Sep;38(9):1141-1147.

doi: 10.1007/s44211-022-00153-z. Epub 2022 Jul 10.

Authors

Minchuan Luo¹, Hiroshi Yukawa^{2

3

4

5

6}, Yoshinobu Baba^{7

8

9}

Affiliations

¹ Nanobio Analytical Chemistry, Biomolecular Chemistry, Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.
² Nanobio Analytical Chemistry, Biomolecular Chemistry, Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. h.yukawa@nanobio.nagoya-u.ac.jp.
³ Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. h.yukawa@nanobio.nagoya-u.ac.jp.
⁴ Institute of Quantum Life Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, Anagawa, Inageku, Chiba, 263-8555, Japan. h.yukawa@nanobio.nagoya-u.ac.jp.
⁵ Advanced Analytical and Diagnostic Imaging Center (AADIC)/Medical Engineering Unit (MEU), Nagoya University Institute for Advanced Research, B3 Unit, Tsurumai 65, Showa-ku, Nagoya, 466-8550, Japan. h.yukawa@nanobio.nagoya-u.ac.jp.
⁶ Development of Quantum-Nano Cancer Photoimmunotherapy for Clinical Application of Refractory Cancer, Nagoya University, Tsurumai 65, Showa-ku, Nagoya, 466-8550, Japan. h.yukawa@nanobio.nagoya-u.ac.jp.
⁷ Nanobio Analytical Chemistry, Biomolecular Chemistry, Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. babaymtt@chembio.nagoya-u.ac.jp.
⁸ Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. babaymtt@chembio.nagoya-u.ac.jp.
⁹ Institute of Quantum Life Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, Anagawa, Inageku, Chiba, 263-8555, Japan. babaymtt@chembio.nagoya-u.ac.jp.

PMID: 35819752
DOI: 10.1007/s44211-022-00153-z

Abstract

Imaging technology based on novel nanomaterials is burgeoning as a potential tool for exploring various physiological processes. We herein report a fluorescent and magnetic nanoprobe (QMNP-RGD) for bimodal imaging of in vitro tumor cells. The preparation of this multifunctional nanomaterial is divided into three steps. First, commercial quantum dots (QDs) with high fluorescence intensity are covalently modified with an RGD peptide, which can facilitate the tumor cell uptake by α_vβ₃ integrin-induced active recognition. Superparamagnetic iron oxide (SPIO) nanoparticles (NPs) are then capped using a cationic polysaccharide to improve stability. Integration is finally achieved by convenient electrostatic binding. We successfully demonstrated that QMNP-RGD can be efficiently delivered into U87MG cells and used for fluorescence/magnetic resonance (MR) bimodal imaging. Other multimodal probes may be able to be designed for imaging based on this strategy of electrostatic binding.

Keywords: Fluorescence imaging; Iron oxide; Magnetic resonance imaging; Quantum dot; U87MG tumor cell.

PubMed Disclaimer

References

1. Y. Yang, X. Fan, L. Li, Y. Yang, A. Nuernisha, D. Xue, C. He, J. Qian, Q. Hu, H. Chen, J. Liu, W. Huang, ACS Nano 14, 509–521 (2020) - DOI
1. G. Lv, L. Qiu, Y. Sun, K. Li, Q. Liu, Q. Zhao, S. Qin, J. Lin, J. Nucl. Med. 61, 117–122 (2020) - DOI
1. Y. Ho, C. Wu, Q. Jin, C. Lin, P. Chiang, N. Wu, C. Fan, C. Yang, C. Yeh, Biomaterials 232, 119723 (2020) - DOI
1. Z. Zhou, B. Li, C. Shen, D. Wu, H. Fan, J. Zhao, H. Li, Z. Zeng, Z. Luo, L. Ma, C. Tan, Small 16, 2004173 (2020) - DOI
1. F. Jungmann, G.A. Kaissis, S. Ziegelmayer, F. Harder, C. Schilling, H. Yen, K. Steiger, W. Weichert, R. Schirren, I.E. Demir, H. Friess, M.R. Makowski, R.F. Braren, F.K. Lohöfer, Cancers 13, 2069 (2021) - DOI

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Fluorescent/magnetic nano-aggregation via electrostatic force between modified quantum dot and iron oxide nanoparticles for bimodal imaging of U87MG tumor cells

Affiliations

Fluorescent/magnetic nano-aggregation via electrostatic force between modified quantum dot and iron oxide nanoparticles for bimodal imaging of U87MG tumor cells

Authors

Affiliations

Abstract

References

MeSH terms

Substances

Grants and funding

LinkOut - more resources

Full Text Sources