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. 2022:2524:333-346.
doi: 10.1007/978-1-0716-2453-1_27.

Protocols for the Evaluation of a Lymphatic Drug Delivery System Combined with Bioluminescence to Treat Metastatic Lymph Nodes

Ariunbuyan Sukhbaatar  1 , Tetsuya Kodama  2   3
Affiliations

Protocols for the Evaluation of a Lymphatic Drug Delivery System Combined with Bioluminescence to Treat Metastatic Lymph Nodes

Ariunbuyan Sukhbaatar et al. Methods Mol Biol. 2022.

Abstract

Bioluminescence (BL) imaging is a powerful non-invasive imaging modality widely used in a broad range of biological disciplines for many types of measurements. The applications of BL imaging in biomedicine are diverse, including tracking bacterial progression, research on gene expression patterns, monitoring tumor cell growth/regression or treatment responses, determining the location and proliferation of stem cells, and so on. It is particularly valuable when studying tissues at depths of 1 to 2 cm in mouse models during preclinical research. Here we describe the protocols for the therapeutic evaluation of a lymphatic drug delivery system (LDDS) using an in vivo BL imaging system (IVIS) for the treatment of metastatic lymph nodes (LNs) with 5-fluorouracil (5-FU). The LDDS is a method that directly injects anticancer drugs into sentinel LNs (SLNs) and delivers them to their downstream LNs. In the protocol, we show that metastases in the proper axillary LN (PALN) are induced by the injection of luciferase-expressing tumor cells into the subiliac LN (SiLN) of MXH10/Mo-lpr/lpr (MXH10/Mo/lpr) mice. 5-FU is injected using the LDDS into the accessory axillary LN (AALN) to treat tumor cells in the PALN after the tumor cell growth is confirmed in the PALN. The tumor growth and therapeutic effects are evaluated by IVIS. This method can be used to evaluate tumor growth and efficacy of anticancer drugs/particles, radiotherapy, surgery, and/or a combination of these methods in various experimental procedures in the oncology field.

Keywords: 5-FU; Chemotherapy; Drug delivery; Intranodal inoculation; Lymph node; Lymph node metastatic; Lymphatic drug delivery system; Mouse model; Ultrasound.

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References

    1. Levin M, Björnheden T, Evaldsson M, Walenta S, Wiklund O (1999) A bioluminescence method for the mapping of local ATP concentrations within the arterial wall, with potential to assess the in vivo situation. Arterioscler Thromb Vasc Biol 19(4):950–958. https://doi.org/10.1161/01.atv.19.4.950 - DOI - PubMed
    1. Rehemtulla A, Stegman LD, Cardozo SJ, Gupta S, Hall DE, Contag CH, Ross BD (2000) Rapid and quantitative assessment of cancer treatment response using in vivo bioluminescence imaging. Neoplasia 2(6):491–495. https://doi.org/10.1038/sj.neo.7900121 - DOI - PubMed - PMC
    1. Hoffman RM (2001) Visualization of GFP-expressing tumors and metastasis in vivo. BioTechniques 30(5):1016–1022., 1024–1016. https://doi.org/10.2144/01305bi01 - DOI - PubMed
    1. Contag CH, Bachmann MH (2002) Advances in in vivo bioluminescence imaging of gene expression. Annu Rev Biomed Eng 4:235–260. https://doi.org/10.1146/annurev.bioeng.4.111901.093336 - DOI - PubMed
    1. Sato A, Klaunberg B, Tolwani R (2004) In vivo bioluminescence imaging. Comp Med 54(6):631–634 - PubMed

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