Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Sep;389(3):385-407.
doi: 10.1007/s00441-022-03666-1. Epub 2022 Jul 13.

Expression analysis of peptidergic enteroendocrine cells in the silkworm Bombyx mori

Affiliations

Expression analysis of peptidergic enteroendocrine cells in the silkworm Bombyx mori

Ladislav Roller et al. Cell Tissue Res. 2022 Sep.

Abstract

Enteroendocrine cells (ECs) in the insect midgut respond to physiological changes in the intestine by releasing multiple peptides to control food intake, gastrointestinal activity and systemic metabolism. Here, we performed a comprehensive mapping of ECs producing different regulatory peptides in the larval midgut of Bombyx mori. In total, we identified 20 peptide genes expressed in different ECs in specific regions of the midgut. Transcript-specific in situ hybridisation combined with antibody staining revealed approximately 30 subsets of ECs, each producing a unique peptide or a combination of several different peptides. Functional significance of this diversity and specific roles of different enteroendocrine peptides are largely unknown. Results of this study highlight the importance of the midgut as a major endocrine/paracrine source of regulatory molecules in insects and provide important information to clarify functions of ECs during larval feeding and development.

Keywords: Co-expression; Endocrine cells; Immunohistochemistry; In situ hybridisation; Midgut; Regulatory peptides.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Amcheslavsky A, Song W, Li Q, Nie Y, Bragatto I, Ferrandon D, Perrimon N, Ip YT (2014) Enteroendocrine cells support intestinal stem-cell-mediated homeostasis in Drosophila. Cell Rep 9:32–39 - PubMed - PMC - DOI
    1. Andriès JC, Tramu G (1985) Ultrastructural and immunohistochemical study of endocrine cells in the midgut of the cockroach Blaberus craniifer (Insecta, Dictyoptera). Cell Tissue Res 240:323–332 - DOI
    1. Antonova Y, Arik AJ, Moore W, Riehle MR, Brown MR (2012) Insulin-like peptides: structure, signalling and function. 63–92. In Gilbert LI ed Insect Endocrinology New York, Elsevier Academic Press
    1. Beehler-Evans R, Micchelli CA (2015) Generation of enteroendocrine cell diversity in midgut stem cell lineages. Development 142:654–664 - PubMed - PMC - DOI
    1. Begum K, Li B, Beeman RW, Park Y (2009) Functions of ion transport peptide and ion transport peptide-like in the red flour beetle Tribolium castaneum. Insect Biochem Mol Biol 39(10):717–725 - PubMed - DOI

Substances

LinkOut - more resources