Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Nov;78(11):4579-4588.
doi: 10.1002/ps.7077. Epub 2022 Jul 29.

New insights into cypermethrin insecticide resistance mechanisms of Culex pipiens pallens by proteome analysis

Chongxing Zhang  1 , Xiuxia Guo  1 , Tao Li  2 , Peng Cheng  1 , Maoqing Gong  1
Affiliations

New insights into cypermethrin insecticide resistance mechanisms of Culex pipiens pallens by proteome analysis

Chongxing Zhang et al. Pest Manag Sci. 2022 Nov.

Abstract

Background: Due to the development of insecticide resistance in mosquitoes, with worldwide mosquito-borne diseases resurgence in recent years, recent advances in proteome technology have facilitated a proteome-wide analysis of insecticide resistance-associated proteins in mosquitoes. Understanding the complexity of the molecular basis of insecticide resistance mechanisms employed by mosquitoes will help in designing the most effective and sustainable mosquito control methods.

Results: After 30 generations, insecticide-selected strains showed elevated resistance levels to the cypermethrin used for selection. Proteome data allowed the detection of 2892 proteins, of which 2885 differentially expressed proteins (DEPs) achieved quantitative significances in four stages (egg, larvae, pupae, adult) of Culex pipiens pallens cypermethrin-resistant strain as compared to the susceptible strain. Among them, a significant enrichment of proteins, including cuticular proteins, enzymes involved in the detoxification (cytochrome P450, glutathione S-transferases, esterase, ATP-binding cassette) and some biological pathways (oxidative phosphorylation, hippo signalling) that are potentially involved in cypermethrin resistance, was observed. Thirty-one representative DEPs (cytochrome P450, glutathione S-transferase, cuticle protein) during Cx. pipiens pallens developmental stages were confirmed by a parallel reaction monitoring strategy.

Conclusions: The present study confirmed the power of isobaric tags for relative and absolute quantification for identifying concomitantly quantitative proteome changes associated with cypermethrin in Cx. pipiens pallens. Proteome analysis suggests that proteome modifications can be selected rapidly by cypermethrin, and multiple resistance mechanisms operate simultaneously in cypermethrin-resistance of Cx. pipiens pallens, Our results interpret that an up-regulated expression of proteins and enzymes like cytochrome P450, glutathione S-transferases, esterase etc. has an impact in insecticide resistance. Previously neglected penetration resistance (cuticular proteins) may play an important role in the adaptive response of Cx. pipiens pallens to insecticides. This information may serve as a basis for future work concerning the possible role of these proteins in cypermethrin resistance in mosquito Cx. pipiens pallens. © 2022 Society of Chemical Industry.

Keywords: Culex pipiens pallens; cypermethrin resistance; isobaric tags for relative and absolute quantification; mosquito.

PubMed Disclaimer

References

REFERENCES

    1. Thai KT and Anders KL, The role of climate variability and change in the transmission dynamics and geographic distribution of dengue. Exp Biol Med (Maywood) 236:944-954 (2011).
    1. Weaver SC and Reisen WK, Present and future arboviral threats. Antiviral Res 85:328-345 (2010).
    1. Hemingway J and Ranson H, Insecticide resistance in insect vectors of human disease. Annu Rev Entomol 45:371-391 (2000).
    1. Pennington MJ, Prager SM, Walton WE and Trumble JT, Culex quinquefasciatus larval microbiomes vary with instar and exposure to common wastewater contaminants. Scientific Reports 6:21969 (2016).
    1. Butler D, Mosquitoes score in chemical war. Nature 475:19 (2011).

MeSH terms

LinkOut - more resources