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. 2022:2511:183-200.
doi: 10.1007/978-1-0716-2395-4_14.

Antibody-Based Affinity Capture Combined with LC-MS Analysis for Identification of COVID-19 Disease Serum Biomarkers

Paul C Guest  1 , Hassan Rahmoune  2
Affiliations

Antibody-Based Affinity Capture Combined with LC-MS Analysis for Identification of COVID-19 Disease Serum Biomarkers

Paul C Guest et al. Methods Mol Biol. 2022.

Abstract

Blood serum or plasma proteins are potentially useful in COVID-19 research as biomarkers for risk prediction, diagnosis, stratification, and treatment monitoring. However, serum protein-based biomarker identification and validation is complicated due to the wide concentration range of these proteins, which spans more than ten orders of magnitude. Here we present a combined affinity purification-liquid chromatography mass spectrometry approach which allows identification and quantitation of the most abundant serum proteins along with the nonspecifically bound and interaction proteins. This led to the reproducible identification of more than 100 proteins that were not specifically targeted by the affinity column. Many of these have already been implicated in COVID-19 disease.

Keywords: Affinity capture; Antibody; Biomarker; COVID-19; LC-MS; Liquid chromatography; Mass spectrometry; SARS-CoV-2.

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