doi: 10.1007/BF00396389.
Polyploidization of G2M phase cells separated from aerobically and anaerobically grown Ehrlich ascites tumor cells
- PMID: 3584220
- PMCID: PMC12248354
- DOI: 10.1007/BF00396389
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Polyploidization of G2M phase cells separated from aerobically and anaerobically grown Ehrlich ascites tumor cells
J Cancer Res Clin Oncol.
1987.
Abstract
G2-enriched fractions of Ehrlich ascites tumor cells (up to 80%-85% G2 cells) separated from anaerobically and aerobically cultured asynchronous populations by centrifugal elutriation revealed the same growth characteristics after recultivation under standard conditions: a significant proportion of cells with increased DNA (DNA content greater than 4C) emerged. Interruption of DNA synthesis by deprivation of oxygen may account for polyploidization (over-replication) of DNA but other mechanisms must be taken into consideration.
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