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. 2022 Jul 16;12(1):12192.
doi: 10.1038/s41598-022-16507-6.

Effect of tempol and straw size on rooster sperm quality and fertility after post-thawing

Abouzar Najafi  1 , Mahdieh Mehdipour  2 , Hossein Mohammadi  3 , Zohreh Mehdipour  4 , Behzad Khorrami  5 , Mahdi Nazari  2
Affiliations

Effect of tempol and straw size on rooster sperm quality and fertility after post-thawing

Abouzar Najafi et al. Sci Rep. .

Abstract

The aim of the present study was to investigate the effects of tempol and straw size on rooster sperm post-thaw quality and fertility. Rooster semen was cryopreserved in Lake extender containing 0 (control), 5, 10, 15 and 20 μM tempol (in two different straw size, 0.25 and 0.5). The percentage of total and progressive sperm motility, VAP and VSL increased in the 10 µM tempol group. Moreover, 10 µM tempol led to lower ROS compared to other groups. The lowest percentage of apoptotic-like changes was detected when the extender was treated with 10 µM of tempol. The minimum ROS was observed in the group treated with 0.5 straw size. Straw size did not have any significantly effect on GPx and SOD activities and TAC of frozen-thawed sperm. The highest significant percentage of fertility and hatching rate were observed in 10 µM of tempol. The results of the present study showed that supplementation of the Lake cryopreservation medium with 10 µM tempol improved cryo-survival. Also, the results of the present study suggested that Lake cryopreservation medium with 0.5-ml straw may perhaps be an appropriate method to improve the quality and fertility post-thawed rooster sperm.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Membrane integrity (a), Total abnormality (b), mitochondrial activity (c), ROS (d), SOD (e), GPx (f) and TAC (g) after thawing samples cryopreserved with the different tempol concentrations. Different letters indicate that treatments differ P < 0.05.
See this image and copyright information in PMC

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