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Comparative Study
. 2022 Sep;104(1):115745.
doi: 10.1016/j.diagmicrobio.2022.115745. Epub 2022 Jun 18.

Comparison of ceftazidime-avibactam susceptibility testing methods against OXA-48-like carrying Klebsiella blood stream isolates

Burcu Isler  1 , Cansel Vatansever  2 , Berna Özer  2 , Güle Çınar  3 , Abdullah Tarık Aslan  4 , Adam Stewart  5 , Peter Simos  6 , Caitlin Falconer  5 , Michelle J Bauer  5 , Brian Forde  5 , Patrick Harris  5 , Funda Şimşek  7 , Necla Tülek  8 , Hamiyet Demirkaya  9 , Şirin Menekşe  10 , Halis Akalin  11 , İlker İnanç Balkan  12 , Mehtap Aydın  13 , Elif Tükenmez Tigen  14 , Safiye Koçulu Demir  15 , Mahir Kapmaz  16 , Şiran Keske  17 , Özlem Doğan  2 , Çiğdem Arabacı  18 , Serap Yağcı  19 , Gülşen Hazırolan  20 , Veli Oğuzalp Bakır  21 , Mehmet Gönen  22 , Neşe Saltoğlu  12 , Alpay Azap  3 , Özlem Azap  9 , Murat Akova  4 , Önder Ergönül  23 , David L Paterson  5 , Füsun Can  23
Affiliations
Comparative Study

Comparison of ceftazidime-avibactam susceptibility testing methods against OXA-48-like carrying Klebsiella blood stream isolates

Burcu Isler et al. Diagn Microbiol Infect Dis. 2022 Sep.

Abstract

Ceftazidime-avibactam exhibits good in vitro activity against carbapenem resistant Klebsiella carrying OXA-48-like enzymes. We tested two hundred unique carbapenem resistant Klebsiella blood stream isolates (71% with single OXA-48-like carbapenemases, including OXA-48, n = 62; OXA-232, n = 57; OXA-244, n = 17; OXA-181, n = 5) that were collected as part of a multicentre study against ceftazidime-avibactam using Etest (bioMérieux, Marcyl'Étoile, France), 10/4 μg disc (Thermo Fisher) and Sensititre Gram Negative EURGNCOL Plates (Lyophilized panels, Sensititre, Thermo Fisher) with the aim of comparing the performances of the Etest and disc to that of Sensititre. Ceftazidime-avibactam MIC50/90 was 2/>16 mg/L for the entire collection and was 2/4 mg/L for single OXA-48-like producers. Categorical and essential agreements between the Etest and Sensititre were 100% and 97%, respectively. Categorical agreement between the disc and Sensititre was 100%. Etest and 10/4 μg discs are suitable alternatives to Sensititre for ceftazidime-avibactam sensitivity testing for OXA-48-like producers.

Keywords: Carbapenem resistant Klebsiella; Ceftazidime-avibactam; Etest; OXA-232; OXA-48-like; Sensititre plate.

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Conflict of interest statement

Declaration of competing interest Dr. Paterson reports research grants from Merck, Pfizer and Shionogi. David Paterson has received honoraria for advisory board membership from Merck, Pfizer, Shionogi, GSK, QPex, Entasis, VenatoRx, BioMerieux, and Accelerate. Dr Harris has received research grants from Sandoz, Merck/MSD and Shionogi, speaker's fees from Pfizer and honoraria for advisory board membership from Merck and Sandoz, paid to the University of Queensland. All others have no conflict of interest to declare.

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