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. 2022 Apr 7;10(7):2381-2389.
doi: 10.1002/fsn3.2846. eCollection 2022 Jul.

Antinociceptive activity of the Caesalpinia eriostachys Benth. ethanolic extract, fractions, and isolated compounds in mice

Hyun-Yong Kim  1 , Hee Jung Lee  2 , Guanglei Zuo  1 , Seung Hwan Hwang  1 , Jeong Seok Park  3 , Jae Seung Hong  3 , Kang Hyuk Kim  1 , Silvia Soto Montero  4 , Dong-Keun Yi  5 , Jeong Tae Lee  6 , Hong-Won Suh  2 , Soon Sung Lim  1   7
Affiliations

Antinociceptive activity of the Caesalpinia eriostachys Benth. ethanolic extract, fractions, and isolated compounds in mice

Hyun-Yong Kim et al. Food Sci Nutr. .

Abstract

Caesalpinia eriostachys Benth. (CE) is native to the Mexico and multiple effects have been observed from several plants belonging to the same family. CE was subjected to extraction with 95% ethanol, and the components were isolated through column chromatography. The structure of the compound was elucidated based on nuclear magnetic resonance (NMR) spectral data, electron ionization-mass (EI-MS) spectroscopy, and liquid chromatography-mass (LC-MS) spectroscopy. In vivo antinociceptive studies were conducted using writhing, 5% formalin, tail-flick, hot-plate, and von Frey filament tests. The ethanolic extract showed a significant effect in the acetic acid-induced pain model and nociceptive behavior in the formalin model (second phase). In hot-plate test and tail-flick test, the results showed no difference compared to the control group. The results suggest that the ethanolic extract may act peripherally to reduce pain. In the streptozotocin (STZ)-induced pain model, the ethanolic extract showed significant effect in the von Frey test model. The n-Hex (Hexane) and MC (Methylene chloride) fractions and isolated compounds, ellagic acid and agathisflavone, showed increased effect. Based on these results, we confirmed that the CE ethanolic extract and their compounds, ellagic acid and agathisflavone, have antinociceptive effect on diabetes mellitus-induced pain. Furthermore, the results of this study might be valuable for identifying compounds with antinociceptive activity from natural products.

Keywords: Caesalpinia eriostachys Benth; agathisflavone; antinociceptive effect; pain; phytochemical analysis.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

FIGURE 1
FIGURE 1
Chemical structure of ellagic acid and agathisflavon
FIGURE 2
FIGURE 2
Chromatogram of the CE extract and fractions. The groups are shown as follows: (a) CE ethanolic extract and fractions [(b) Hexane (n‐Hex), (c) Methylene chloride (MC), (d) Ethyl acetate (EtOAc), (e) Butanol (n‐BuOH), and (f) water]
FIGURE 3
FIGURE 3
Effect of CE ethanolic extract in the hot‐plate test (a) and tail‐flick test (b). Data are expressed as mean ± SD; n = 5 mice per group
FIGURE 4
FIGURE 4
Effect of ethanolic extract of CE in the acetic acid‐induced abdominal writhing test (a) and formalin test (b) in mice. Data are expressed as mean ± SD; n = 5 mice per group (* p < .05, ** p < .01, *** p < .001 versus. control group)
FIGURE 5
FIGURE 5
Effect of CE ethanolic extract, according to the von Frey filament test, on the streptozotocin‐induced mouse model of diabetic peripheral neuropathic pain. Data are expressed as mean ± SD; n = 5 mice per group (## p < .01 versus. intact group; * p < .05, ** p < .01 versus. control group)
FIGURE 6
FIGURE 6
Effect of CE ethanolic extract fractionations, according to the von Frey filament test, on the streptozotocin‐induced mouse model of diabetic peripheral neuropathic pain. Data are expressed as mean ± SD; n = 5 mice per group (## p < .01 versus. intact group; * p < .05, ** p < .001 versus. control group)
FIGURE 7
FIGURE 7
Effect of isolated compounds from CE ethanolic extract, according to the von Frey filament test, on the streptozotocin‐induced mouse model of diabetic peripheral neuropathic pain. Data are expressed as mean ± SD; n = 5 mice per group (## p < .01 versus. intact group; * p < .05 versus. control group)
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