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. 2022 Jul 12:10:e13684.
doi: 10.7717/peerj.13684. eCollection 2022.

Prevalence of HPV genotypes and assessment of their clinical relevance in laryngeal squamous cell carcinoma in a northeastern state of Brazil-a retrospective study

Charlles Brito  1 , Rachel D Cossetti  2   3 , Diego Agra de Souza  3 , Marcos Catanha  3 , Pablo de Matos Monteiro  4 , Flavia Castello Branco Vidal  1   5
Affiliations

Prevalence of HPV genotypes and assessment of their clinical relevance in laryngeal squamous cell carcinoma in a northeastern state of Brazil-a retrospective study

Charlles Brito et al. PeerJ. .

Abstract

Background: A high prevalence and incidence of head and neck tumors make Brazil the country with the third-highest number of cases of these malignant neoplasms. The main risk factors are smoking and alcohol consumption; however, cases related to the human papillomavirus (HPV) have tripled in number, demonstrating a changing disease profile. Studies have reported the prevalence of HPV in laryngeal squamous cell carcinoma (LSCC) to vary between 8% and 83%. The role of HPV as an important causative factor in LSCC remains unclear.

Methods: This retrospective study included 82 patients with LSCC diagnosed between 2014 and 2019 at two oncology hospitals in São Luís, Brazil. Sociodemographic and clinical data, and the histopathologic characteristics of the tumors, were collected directly from medical records. Genetic material was extracted from paraffin-embedded samples using nested polymerase chain reaction (PCR) and automated sequencing for HPV detection and genotyping. The results by social and clinicopathologic variables were then compared using the chi-squared test and multivariate analysis.

Results: Sociodemographic analysesdemonstrated that most patients were men (87.8%), brown-skinned (75.6%), and resident in the state capital (53.7%). They generally had a poor education status (53.7%), having only an elementary school education (completed/incomplete), and 51.2% were self-employed in occupations such as farming or fishing. Smoking and alcohol consumption habits were observed in approximately half the patients. With respect to clinical characteristics, 39% of patients exhibited T1/T2 staging, 51.2% had no distant metastasis, and 30.5% had lymph node invasion. HPV DNA was detected in half the samples (50%), with the high oncogenic type 16 being the most prevalent. There was no significant relationship observed between the economic, educational, occupational with the HPV LSCC in the presented data, although multivariate analysis demonstrated that HPV DNA was more likely to be present in T3-T4 tumors (p = 0.002).

Keywords: Laryngeal neoplasms; Papillomavirus infections; Prevalence; Squamous cell carcinoma of head and neck.

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Conflict of interest statement

The authors declare there are no competing interests.

Figures

Figure 1
Figure 1. Distribution of laryngeal cancer patients (n = 41) stratified by HPV type.
See this image and copyright information in PMC

References

    1. Abreu ALP, Souza RP, Gimenes F, Consolaro MEL. A review of methods for detect human Papillomavirus infection. Virology Journal. 2012;9:262. doi: 10.1186/1743-422X-9-262. - DOI - PMC - PubMed
    1. Amit M, Ilana K, Avraham SP, Binenbaum Y, Bachar G, Billan S, Zaarura S, Czerninski R, Bar-Tov M, Maly A, Akrish S, Gil Z. Trends in human papillomavirus-related oropharyngeal cancer in Israel. Head & Neck. 2016;38(Suppl 1):E274–E278. doi: 10.1002/hed.23985. - DOI - PubMed
    1. Brazilian Institute of Geography and Statistics (IBGE) Índice de Desenvolvimento Humano. 2010. https://biblioteca.ibge.gov.br/visualizacao/livros/liv49230.pdf https://biblioteca.ibge.gov.br/visualizacao/livros/liv49230.pdf
    1. Bruni L, Albero G, Serrano B, Mena M, Collado JJ, Gómez D, Muñoz J, Bosch FX, de Sanjosé S. https://hpvcentre.net/statistics/reports/XWX.pdf ICO/IARC Information Centre on HPV and Cancer (HPV Information Centre). Human Papillomavirus and Related Diseases in the World. Summary Report 22 October 2021. 2016
    1. Carvalho AMAS, de Brito DS, Cunha APA, Monteiro PdeM, Ferreira MB, de Assis DSFR, Bentivi JO, de Andrade MS, Vidal FCB, Filho NS. Recurrent respiratory papillomatosis: clinical characteristics and viral genotyping in a Brazilian population. Revista do Instituto de Medicina Tropical de São Paulo. 2021;63:e63. doi: 10.1590/s1678-9946202163063. - DOI - PMC - PubMed

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