Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jul 5:2022:9619626.
doi: 10.1155/2022/9619626. eCollection 2022.

Relationship of the Circulating Endothelial Progenitor Cells to the Severity of a Coronary Artery Lesion in Unstable Angina

Cheng Xiao  1 , Lixiang Liu  2 , Xi Li  3 , Xiaoan Yang  4 , Hanxiong Liu  5
Affiliations

Relationship of the Circulating Endothelial Progenitor Cells to the Severity of a Coronary Artery Lesion in Unstable Angina

Cheng Xiao et al. Cardiol Res Pract. .

Abstract

The number and function of circulating endothelial progenitor cells (EPCs) decreased in stable coronary artery disease. Nevertheless, there were few studies that explored the variation of EPC and the relationship with the severity of coronary artery lesions in unstable angina (UA). Therefore, this leaves an area for the investigation of the difference in the number and activity of circulating EPCs and the relationship with the Gensini score in unstable angina. Fluorescence-activated cell sorter analysis, as well as DiI-acLDL and lectin fluorescent staining measure the number of circulating EPCs. The transwell chamber assay and MTT were evaluated by the migration and proliferation of circulating EPCs. In addition, the flow-mediated dilation (FMD), Gensini score, and IL-6 levels in plasma were determined. We found that UA patients had the higher number and lower function of circulating EPCs. With the increase in severity of coronary artery lesions, the migration and proliferation of EPCs were decreased. Moreover, the function of the circulating EPCs was negatively associated with severity of coronary artery lesions in unstable angina. In addition, UA patients presented elevated IL-6, which was negatively correlated with the function of circulating EPCs and FMD and positively correlated with the severity of coronary artery lesions evaluated by the Gensini score. These findings revealed the decline in the function of circulating EPCs was associated with the severity of coronary artery disease, which may be related to systemic inflammation.

PubMed Disclaimer

Conflict of interest statement

The authors declare that there are no conflicts of interest regarding the publication of this paper.

Figures

Figure 1
Figure 1
The number and function of circulating EPCs between the two groups. The number of circulating EPCs was detected by determining the number of CD34+/KDR+ cells per 100 peripheral blood mononuclear cells and then by examining the numbers of DiI-acLDL/lectin double-positive cells (a), (b) The results of migration and proliferation assays (c), (d) Statistical significance was evaluated using Student's t-test or analysis of variance.∗P < 0.05
Figure 2
Figure 2
The number and function of circulating EPCs between three subgroups. As the Gensini score increased, the number of circulating EPCs was decreased (a), (b) and the migration and proliferation of circulating EPCs were also declined. (c), (d) Statistical significance was evaluated using Student's t-test or analysis of variance.
Figure 3
Figure 3
Correlation between FMD and the Gensini score and EPC function. FMD was positively correlated with EPC migration (a) and proliferation (b) In addition, the Genisini score was inversely related to EPC migration (c) and proliferation (d) And the Genisini score was inversely related to FMD (e) Univariate correlations were calculated using Pearson's coefficient (r).
Figure 4
Figure 4
Correlation between the function of circulating EPCs or FMD or the Gensini score and IL-6. EPC migration (a) proliferation (b) and FMD (c) were inversely related to IL-6. In addition, the Gensini score was positively correlated with IL-6 (d) Univariate correlations were calculated using Pearson's coefficient (r).
See this image and copyright information in PMC

References

    1. Charach L., Blatt A., Jonas M., et al. Using the gensini score to estimate severity of STEMI, NSTEMI, unstable angina, and anginal syndrome. Medicine . 2021;100(41) doi: 10.1097/MD.0000000000027331.e27331 - DOI - PMC - PubMed
    1. Chen S., Shen Y., Liu Y., et al. Impact of glycemic control on the association of endothelial dysfunction and coronary artery disease in patients with type 2 diabetes mellitus. Cardiovascular Diabetology . 2021;20(1) doi: 10.1186/s12933-021-01257-y. - DOI - PMC - PubMed
    1. Chou R., Chen C., Chen I., et al. Trimethylamine N-oxide, circulating endothelial progenitor cells, and endothelial function in patients with stable Angina. Scientific Reports . 2019;9(1):p. 4249. doi: 10.1038/s41598-019-40638-y. - DOI - PMC - PubMed
    1. Fanola C. L., Morrow D. A., Cannon C. P., et al. Interleukin‐6 and the risk of adverse outcomes in patients after an acute coronary syndrome: observations from the SOLID‐TIMI 52 (stabilization of plaque using darapladib—thrombolysis in myocardial infarction 52) trial. Journal of American Heart Association . 2017;6(10) doi: 10.1161/jaha.117.005637. - DOI - PMC - PubMed
    1. George J. Circulating endothelial progenitor cells in patients with unstable angina: association with systemic inflammation. European Heart Journal . 2004;25(12):1003–1008. doi: 10.1016/j.ehj.2004.03.026. - DOI - PubMed

LinkOut - more resources