Potential tradeoffs between effects of arbuscular mycorrhizal fungi inoculation, soil organic matter content and fertilizer application in raspberry production

Abstract

Ecological intensification has been proposed as an alternative paradigm for intensive agriculture to boost yield sustainably through utilizing ecosystem services. A prerequisite to achieving this is to understand the relations between multiple ecosystem services and production, while taking growth conditions such as nutrient availability into consideration. Here, we conducted a pot-field experiment to study the interactive effects of soil organic matter (SOM) content and arbuscular mycorrhizal fungi (AMF) inoculation on the production of raspberry (Rubus idaeus L.) under four levels of fertilizer application. Raspberry flower number, fruit number and yield only significantly increased with fertilizer inputs but were not impacted by SOM content or AMF inoculation. Fruit set and single berry weight were influenced by both SOM content and AMF inoculation, in complex three-way interactions with fertilizer application. Fruit set of AMF inoculated plants increased with fertilizer inputs in low SOM soils, but decreased with fertilizer inputs under high SOM soils, with the highest fruit set occurring at no fertilizer inputs. In low SOM soils, the relation between single berry weight and fertilizer application was more pronounced in inoculated plants than in non-inoculated plants, while in high SOM soils the relative benefits of AMF inoculation on single berry weight decreased with increasing fertilizer inputs. We attribute the lack of effects of AMF inoculation and SOM content on flower number, fruit number and yield mainly to potential tradeoffs between the experimental variables that all influence resource uptake by plant root systems. Our results suggest that potentially beneficial effects of AMF and SOM can be offset by each other, probably driven by the dynamic relations between AMF and the host plants. The findings reveal fundamental implications for managing AMF inoculation and SOM management simultaneously in real-world agricultural systems.

Fig 1

Effects of fertilizer application rates on flower number (a), fruit number (b) and yield (c) per plant. Graphs show conditional partial regression plots based on the minimum adequate models. Shadings show the 95% confidence interval, and points represent partial residuals.

Fig 2. Interactive effects of AMF inoculation, SOM and fertilizer application rates on fruit set per plant.

Graphs show conditional partial regression plots based on the minimum adequate model; shadings show the 95% confidence interval, and points represent partial residuals.

Fig 3. Interactive effects of AMF inoculation, SOM and fertilizer application rates on average single berry weight (g) per plant.

Graphs show conditional partial regression plots based on the minimum adequate model; shadings show the 95% confidence interval, and points represent partial residuals.