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. 2022 Jul 19;204(8):503.
doi: 10.1007/s00203-022-03134-y.

Purification, biochemical characterization and fibrinolytic potential of proteases produced by bacteria of the genus Bacillus: a systematic literature review

Arthur Hipólito Pereira Leite  1 , Ítalo Henrique Alves da Silva  2 , Lorenzo Pastrana  3 , Thiago Pajeú Nascimento  4 , Adriana Maria da Silva Telles  2 , Ana Lúcia Figueiredo Porto  5
Affiliations

Purification, biochemical characterization and fibrinolytic potential of proteases produced by bacteria of the genus Bacillus: a systematic literature review

Arthur Hipólito Pereira Leite et al. Arch Microbiol. .

Abstract

Thrombosis is a hematological disorder characterized by the formation of intravascular thrombi, which contributes to the development of cardiovascular diseases. Fibrinolytic enzymes are proteases that promote the hydrolysis of fibrin, promoting the dissolution of thrombi, contributing to the maintenance of adequate blood flow. The characterization of new effective, safe and low-cost fibrinolytic agents is an important strategy for the prevention and treatment of thrombosis. However, the development of new fibrinolytics requires the use of complex methodologies for purification, physicochemical characterization and evaluation of the action potential and toxicity of these enzymes. In this context, microbial enzymes produced by bacteria of the Bacillus genus are promising and widely researched sources to produce new fibrinolytics, with high thrombolytic potential and reduced toxicity. Thus, this review aims to provide a current and comprehensive understanding of the different Bacillus species used for the production of fibrinolytic proteases, highlighting the purification techniques, biochemical characteristics, enzymatic activity and toxicological evaluations used.

Keywords: Bacillus; Biochemical characterization; Fibrinolytic enzymes; Purification; Thrombolytic activity.

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References

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