Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2022 Jul 19;7(1):244.
doi: 10.1038/s41392-022-01109-5.

Back to pluripotency: fully chemically induced reboot of human somatic cells

Lucas Lange  1   2 , Miguel A Esteban  1   3 , Axel Schambach  4   5   6
Affiliations
Comment

Back to pluripotency: fully chemically induced reboot of human somatic cells

Lucas Lange et al. Signal Transduct Target Ther. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Schematic of chemically induced reprogramming of human somatic cells into human chemically induced pluripotent stem cells (hCiPSC). Starting from restricted, terminally differentiated human somatic cells, Guan et al. have established a fully defined, stepwise (stage I to stage IV) reprogramming protocol that relies entirely on small molecules. Disrupted somatic cell identity and epigenetic modulation induced dedifferentiation into a plastic XEN-like state with unlocked restricted potential. Especially the c-Jun N-terminal kinase inhibitor (JNKIN8) was indispensable to downregulate pro-inflammatory pathways that hinder dedifferentiation and generation of cell plasticity. The acquired cell plasticity of the XEN-like stage permitted further reprogramming to stable hCiPSCs. The hCiPSCs can be used for basic research, for example, to further investigate pathways involved in reprogramming, or to screen for cell fate-determining druggable targets that might lead to novel therapeutic options for patients. GMP-compliant, cost-effective reprogramming of human somatic cells into hCiPSCs potentially eases the translation of iPSCs for tailored autologous cell therapy
See this image and copyright information in PMC

Comment on

  • Chemical reprogramming of human somatic cells to pluripotent stem cells.
    Guan J, Wang G, Wang J, Zhang Z, Fu Y, Cheng L, Meng G, Lyu Y, Zhu J, Li Y, Wang Y, Liuyang S, Liu B, Yang Z, He H, Zhong X, Chen Q, Zhang X, Sun S, Lai W, Shi Y, Liu L, Wang L, Li C, Lu S, Deng H. Guan J, et al. Nature. 2022 May;605(7909):325-331. doi: 10.1038/s41586-022-04593-5. Epub 2022 Apr 13. Nature. 2022. PMID: 35418683

References

    1. Guan J, et al. Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature. 2022;605:325–331. doi: 10.1038/s41586-022-04593-5. - DOI - PubMed
    1. Kim JY, Nam Y, Rim YA, Ju JH. Review of the Current Trends in Clinical Trials Involving Induced Pluripotent Stem Cells. Stem Cell Rev. Rep. 2022;18:142–154. doi: 10.1007/s12015-021-10262-3. - DOI - PMC - PubMed
    1. Hou P, et al. Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds. Science. 2013;341:651–654. doi: 10.1126/science.1239278. - DOI - PubMed
    1. Liu J, et al. The oncogene c-Jun impedes somatic cell reprogramming. Nat. Cell Biol. 2015;17:856–867. doi: 10.1038/ncb3193. - DOI - PubMed
    1. Murugan NJ, et al. Acute multidrug delivery via a wearable bioreactor facilitates long-term limb regeneration and functional recovery in adult Xenopus laevis. Sci. Adv. 2022;8:eabj2164. doi: 10.1126/sciadv.abj2164. - DOI - PMC - PubMed

Publication types