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. 2022 Jul 19;8(1):69.
doi: 10.1038/s41421-022-00435-w.

Heterotypic vaccination responses against SARS-CoV-2 Omicron BA.2

Zhenhao Fang #  1   2   3 , Lei Peng #  1   2   3 , Carolina Lucas  4 , Qianqian Lin  1   2   3 , Liqun Zhou  1   2   3   5 , Luojia Yang  1   2   3   6 , Yanzhi Feng  1   2   3   5 , Ping Ren  1   2   3 , Paul A Renauer  1   2   3   6 , Valter S Monteiro  4   5 , Anne M Hahn  7   8 , Jonathan J Park  1   2   3   9 , Xiaoyu Zhou  1   2   3 ; Yale SARS-CoV-2 Genomic Surveillance Initiative; Nathan D Grubaugh  7   8 , Craig B Wilen  4   10 , Sidi Chen  11   12   13   14   15   16   17   18   19
Collaborators, Affiliations

Heterotypic vaccination responses against SARS-CoV-2 Omicron BA.2

Zhenhao Fang et al. Cell Discov. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

We declare that none of the authors have competing financial or non-financial interests related to this study as defined by Nature Portfolio. S.C. is a Founder of EvolveImmune Tx, Cellinfinity Bio, and Chen Consulting, all unrelated to this study. The remaining authors declare no competing interest.

Figures

Fig. 1
Fig. 1. WT and variant-specific LNP-mRNA elicited potent antibody response against Omicron BA.1 and BA.2.
Schematics (a) and spike structure (b) showing Omicron BA.1 and BA.2 mutations distribution in the vaccine design (a) and one protomer of BA.1 spike trimer (b, PDB: 7T9K). c Comparison of antibody response induced by two doses of 1 µg WT, B.1.351 or B.1.617 LNP-mRNA at 21 days interval. Antibody titers were determined by area under curve (AUC) of ELISA titration curves in Supplementary Fig. S1. d Neutralization of Omicron BA.2 pseudovirus by serum samples from mice vaccinated with 1 µg WT, B.1.351 or B.1.617 LNP-mRNA as illustrated in c. The neutralizing titers were quantified by log10 reciprocal IC50 and calculated from titrations in Supplementary Fig. S2. Gating strategy to select GFP positive infected cells is shown in Supplementary Fig. S5. e BA.1 and WT boosters strengthened waning immunity against Omicron BA.1 and BA.2 variants. ELISA antibody titers of samples from mice sequentially vaccinated with two doses of 1 µg WT LNP-mRNA followed by 10 µg WT (WT × 3, n = 5) or Omicron BA.1 (WT × 2 + BA.1, n = 7) LNP-mRNA boosters. The pre-booster groups (day 35 and > 4 month from prime) to receive WT or BA.1 boosters were denoted as WT × 2 (+ WT) and WT × 2 (+ BA.1) respectively. f Neutralization of Omicron BA.2 pseudovirus by plasma samples from mice in e. g Plaque-reduction neutralization (PRNT50) of Omicron BA.2.12.1 infectious virus by plasma samples from mice in e. Related statistical information and all source data can be found in Supplementary Information or Supplementary Dataset S1. Data points of PBS group showed no statistical difference between collection time points and were combined to one group in e–g. Only comparisons between adjacent time points or groups of same time point were shown in e, f. A subset of collected samples were characterized in pseudovirus and infectious virus neutralization assay due to depletion of samples.
See this image and copyright information in PMC

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