Drosophila eugracilis - Akt

Abstract

Gene Model for Akt in the D. eugracilis (DeugGB2) assembly (GCA_000236325.2).

Figure 1.

(A) Synteny of genomic neighborhood of Akt in both D. melanogaster as well as D. eugracilis . Gene arrows pointing in the same direction as Akt in both D. eugracilis and D. melanogaster are on the same strand as Akt ; gene arrows pointing in the opposite direction are on the opposite strand. The thin underlying arrow in D. melanogaster that points to the right indicates Akt is on the + strand and the thin underlying arrow pointing to the left in D. eugracilis indicates Akt is on the – strand. White arrows in D. eugracilis indicate orthology to the corresponding gene in D. melanogaster . CG32855 and sxe2 are nested within an intron of Mhc1 in both species. The gene names given in the D. eugracilis gene arrows indicate the orthologous gene in D. melanogaster , while the locus identifiers are specific to D. eugracilis ; (B) Gene Model in UCSC Track Hub (Raney et al., 2014): The gene model in D. eugracilis (black), Spaln of D. melanogaster Proteins (purple, alignment of refseq proteins from D. melanogaster ), BLAT alignments of NCBI RefSeq Genes (blue, alignment of refseq genes for D. eugracilis ), RNA-Seq from adult females (red), adult males (blue), and mixed embryos (purple) (alignment of Illumina RNAseq reads from D. eugracilis ), and Transcripts (green) including coding regions predicted by TransDecoder and Splice Junctions Predicted by regtools using D. eugracilis RNA-Seq (Chen et al., 2014; PRJNA63469). Splice junctions shown have a minimum read-depth of 10 with 10-49, 50-99, 100-499, 500-999, >1000 supporting reads in blue, green, pink, brown, and red respectively. The custom gene model (User Supplied Track) is indicated in black with exons depicted with boxes and introns with narrow lines (arrows indicate direction of transcription). (C) Dot Plot of amino acid identity for Akt-PC in D. melanogaster ( x -axis) vs. Akt-PC in D. eugracilis ( y -axis). Amino acid number is indicated along the left and bottom; exon number is indicated along the top and right. Each colored rectangle represents an exon. Region 1 indicates a lack of sequence similarity between the two sequences; (D) ROAST Alignments Conservation track within the UCSC Genome Browser. The ROAST Alignments for 28 Drosophila Species track within the UCSC Genome Browser shows the genomic region surrounding the beginning of the Akt-PC isoform in all 28 species shown, demonstrating that the non-canonical start codon for Akt-PC is conserved across the genus.