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Comment
. 2022 Jul 20;20(7):e3001720.
doi: 10.1371/journal.pbio.3001720. eCollection 2022 Jul.

Expanding the epitranscriptome: Dihydrouridine in mRNA

Sameer Dixit  1 , Samie R Jaffrey  1
Affiliations
Comment

Expanding the epitranscriptome: Dihydrouridine in mRNA

Sameer Dixit et al. PLoS Biol. .

Abstract

Nucleotide modifications can markedly influence mRNA processing and metabolism. New studies, one in PLOS Biology, show that approximately 130 yeast mRNAs contain dihydrouridine, a derivative of uridine. Functional studies show that dihydrouridine, in some cases, can affect mRNA splicing.

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Conflict of interest statement

I have read the journal’s policy and the authors of this manuscript have the following competing interests: S.R.J. is scientific advisor to and owns equity in 858 Therapeutics.

Figures

Fig 1
Fig 1. Transcriptome-wide mapping of dihydrouridine with D-seq.
(A) DUS enzymes catalyze the reduction of uridine to dihydrouridine, which comprises the loss of a double bond in the uracil base. Further chemical reduction using sodium borohydride (NaBH4) results in formation of tetrahydrouridine, which results in an increased frequency of reverse transcriptase terminations. (B) Protocol for dihydrouridine profiling in RNA using sodium borohydride treatment in wild-type and DUS KO cells. The sites of reverse transcriptase terminations are mapped. In RNA samples treated with sodium borohydride, the pattern of terminations changes, with an enrichment at sites of tetrahydrouridine. DUS, dihydrouridine synthase; KO, knockout; RT, reverse transcriptase.
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