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Review
. 2022 Dec;23(12):741-759.
doi: 10.1038/s41576-022-00515-3. Epub 2022 Jul 20.

The emerging landscape of spatial profiling technologies

Jeffrey R Moffitt  1   2 , Emma Lundberg  3   4   5   6 , Holger Heyn  7   8
Affiliations
Review

The emerging landscape of spatial profiling technologies

Jeffrey R Moffitt et al. Nat Rev Genet. 2022 Dec.

Abstract

Improved scale, multiplexing and resolution are establishing spatial nucleic acid and protein profiling methods as a major pillar for cellular atlas building of complex samples, from tissues to full organisms. Emerging methods yield omics measurements at resolutions covering the nano- to microscale, enabling the charting of cellular heterogeneity, complex tissue architectures and dynamic changes during development and disease. We present an overview of the developing landscape of in situ spatial genome, transcriptome and proteome technologies, exemplify their impact on cell biology and translational research, and discuss current challenges for their community-wide adoption. Among many transformative applications, we envision that spatial methods will map entire organs and enable next-generation pathology.

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References

    1. Elmentaite, R., Domínguez Conde, C., Yang, L. & Teichmann, S. A. Single-cell atlases: shared and tissue-specific cell types across human organs. Nat. Rev. Genet. 23, 395–410 (2022). - PubMed - DOI
    1. Fawkner-Corbett, D. et al. Spatiotemporal analysis of human intestinal development at single-cell resolution. Cell 184, 810–826 (2021). - PubMed - PMC - DOI
    1. Elmentaite, R. et al. Cells of the human intestinal tract mapped across space and time. Nature 597, 250–255 (2021). - PubMed - PMC - DOI
    1. Asp, M. et al. A spatiotemporal organ-wide gene expression and cell atlas of the developing human heart. Cell 179, 1647–1660 (2019). - PubMed - DOI
    1. Litviňuková, M. et al. Cells of the adult human heart. Nature 588, 466–472 (2020). - PubMed - PMC - DOI

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