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. 2022 Jul;87(2-3):195-205.
doi: 10.1007/s10493-022-00728-5. Epub 2022 Jul 20.

Molecular identification and phylogenetic analysis of spider mites (Prostigmata: Tetranychidae) of Turkey

Emre İnak  1 , Sultan Çobanoğlu  2 , Philippe Auger  3 , Alain Migeon  3
Affiliations

Molecular identification and phylogenetic analysis of spider mites (Prostigmata: Tetranychidae) of Turkey

Emre İnak et al. Exp Appl Acarol. 2022 Jul.

Abstract

The family Tetranychidae includes many agriculturally important species known as spider mites. Their morphological identification is quite difficult due to the tiny size of their taxonomic characters and the requirement for high-level expertise. This may lead to pest misidentification and thus failure in pest management. DNA-based species identification seems to offer an alternative solution to overcome these issues. In the present study, two common molecular markers-Cytochrome oxidase subunit I (COI) and Internal transcribed spacer 2 (ITS2)-were used to identify 10 spider mite species from Turkey. Furthermore, genetic distances for several of them were assessed. Panonychus ulmi and Bryobia kissophila had the lowest (1.1%) and highest (4.5%) intra-specific genetic distances, respectively. In addition, integrative taxonomy allowed to identify Eotetranychus quercicola in Turkey as a new record. The sequences herein obtained will allow rapid species identification using molecular techniques and will contribute to resolve the phylogenetic history of spider mites.

Keywords: Eotetranychus; Genetic distance; Integrative taxonomy; Spider mites; Tetranychidae.

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References

    1. Altunç YE, Akyazı R (2020) Two New Records for Spider Mite Fauna of Turkey, Tetranychus kanzawai Kishida and Eotetranychus rubiphilus Reck (Trombidiformes: Tetranychidae). Turkish J Agric-Food Sci Technol. https://doi.org/10.24925/turjaf.v8i8.1598-1602.2387 - DOI
    1. Arabuli T, Negm MW, Matsuda T, Kitashima Y, Abramishvili T, Akimov IA, Zhovnerchuk OV, Popov SY, Gotoh T (2019) Morphological identification of Amphitetranychus species (Acari: Tetranychidae) with crossbreeding, esterase zymograms and DNA barcode data. PLoS ONE 14(9):e0221951. https://doi.org/10.1371/journal.pone.0221951 - DOI - PubMed - PMC
    1. Auger P, Migeon A (2014) Three new species of Tetranychidae (Acari, Prostigmata) from the French Alps (South-Eastern France). Acarologia 54(1):15–37. https://doi.org/10.1051/acarologia/20142111 - DOI
    1. Auger P, Migeon A, Flechtmann CHW (2003) A new species of Eotetranychus from France (Acari, Prostigmata: Tetranychidae). Zootaxa 206(1):1–7 - DOI
    1. Ben-David T, Melamed S, Gerson U, Morin S (2007) ITS2 sequences as barcodes for identifying and analyzing spider mites (Acari: Tetranychidae). Exp Appl Acarol 41(3):169–181. https://doi.org/10.1007/s10493-007-9058-1 - DOI - PubMed

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