Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jul 11:2022:3729995.
doi: 10.1155/2022/3729995. eCollection 2022.

Study on the Mechanism of circRNA Regulating the miRNA Level in Nephrotic Syndrome

Qianyu Li  1 , Min Yin  1 , Zhiping Zhang  1 , Yuanzhi Yu  1 , Feng Liu  1
Affiliations

Study on the Mechanism of circRNA Regulating the miRNA Level in Nephrotic Syndrome

Qianyu Li et al. Evid Based Complement Alternat Med. .

Retraction in

Abstract

Background: Nephrotic syndrome is an enormous public healthy threaten, which causes a variety of complications and secondary disease; however, the molecular mechanism of nephrotic syndrome remains unclear.

Methods: In our study, RNA-seq were used to test the transcription level of patients with nephrotic syndrome, in order to investigate the interaction of circRNA-miRNA-mRNA in nephrotic syndrome patients.

Results: Consistent with our hypothesis, miRNAs were confirmed to be associated with nephrotic syndrome, majority of their targeting circRNAs downregulated in nephrotic syndrome patients and at the same time, the KEGG pathway analysis found that target genes of the circRNAs bonding miRNAs was highly correlated with the occurrence of kidney diseases.

Conclusion: Thus, we can draw a conclusion that downregulated circRNAs cause miRNA expressing aberrant and then affect the expression level of mRNA, finally leading to the generation of nephrotic syndrome.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
circRNAs in nephrotic syndrome patients are inhibited. (a) Numbers of circRNAs in nephrotic syndrome patients and healthy human. (b) Statistics of the proportion of total circRNAs after RNA-seq. (c) Statistics of the proportion of upregulated circRNAs. (d) Statistics of the proportion of downregulated circRNAs. (e) Numbers of circRNAs produce from one gene (5637 circRNAs generated from 2833 host genes).
Figure 2
Figure 2
Differentially expressed circRNAs and mRNAs cluster analysis. (a) Differentially expressed circRNAs cluster analysis. (b) Scatter plot analysis of different expressed circRNAs. (c) Differentially expressed mRNAs cluster analysis. (d) Scatter plot analysis of differentially expressed mRNAs.
Figure 3
Figure 3
Differentially expressed circRNAs and mRNAs on volcano plots. (a). Volcano plots showed the differential expression of circRNAs between NS and NC. (b). Volcano plots showed the differential expression of mRNAs between NS and NC.
Figure 4
Figure 4
circRNA-miRNA-mRNA co-network: The network shows the 6 circRNAs (yellow), fourteen mRNA (purple), and the 34 miRNAs (green).
Figure 5
Figure 5
The differentially expressed between nephrotic syndrome (NS) patients and healthy (NC). Data are expressed as means ± SD (P < 0.05).
See this image and copyright information in PMC

References

    1. Thoenes W. The pathology of glomerulonephritis. Internist (Berl) . 1989;30(3):127–139. - PubMed
    1. Salmena L., Poliseno L., Tay Y., Kats L., Pandolfi P. A ceRNA hypothesis: the Rosetta Stone of a hidden RNA language? Cell . 2011;146(3):353–358. doi: 10.1016/j.cell.2011.07.014. - DOI - PMC - PubMed
    1. Sellier-Leclerc A. L., Duval A., Riveron S., et al. A humanized mouse model of idiopathic nephrotic syndrome suggests a pathogenic role for immature cells. Journal of the American Society of Nephrology . 2007;18(10):2732–2739. doi: 10.1681/asn.2006121346. - DOI - PubMed
    1. Serino G., Sallustio F., Cox S. N., Pesce F., Schena F. P. Abnormal miR-148b expression promotes aberrant glycosylation of IgA1 in IgA nephropathy. Journal of the American Society of Nephrology . 2012;23(5):814–824. doi: 10.1681/asn.2011060567. - DOI - PMC - PubMed
    1. Chronic Kidney Disease Prognosis Consortium, van der Velde M., Astor B. C., et al. Association of estimated glomerular filtration rate and albuminuria with all-cause and cardiovascular mortality in general population cohorts: a collaborative meta-analysis. Lancet . 2010;375(9731):2073–2081. doi: 10.1016/S0140-6736(10)60674-5. - DOI - PMC - PubMed

Publication types

LinkOut - more resources