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. 2022:2532:95-112.
doi: 10.1007/978-1-0716-2497-5_6.

Assessment of Multiway Interactions with Tri-C

A Marieke Oudelaar  1 , Damien J Downes  2 , Jim R Hughes  2
Affiliations

Assessment of Multiway Interactions with Tri-C

A Marieke Oudelaar et al. Methods Mol Biol. 2022.

Abstract

Tri-C is a chromosome conformation capture (3C) approach that can efficiently identify multiway chromatin interactions with viewpoints of interest. As opposed to pair-wise interactions identified in methods such as Hi-C, 4C, and Capture-C, the detection of multiway interactions allows researchers to investigate how multiple cis-regulatory elements interact together in higher-order structures in single nuclei and address questions regarding structural cooperation between these elements. Here, we describe the procedure for designing and performing a Tri-C experiment.

Keywords: Chromosome conformation capture; Gene regulation; Genome organization; Multiway interactions; cis-regulatory elements.

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References

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